Fertility and Sterility
Volume 92, Issue 6 , Pages 1834-1843 , December 2009

Ectopic and eutopic stromal endometriotic cells have a damaged ceramide signaling pathway to apoptosis

  • Agnieszka Chrobak, Ph.D.

      Affiliations

    • Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland
    • Laboratory of Reproductive Immunology, Institute of Immunology and Experimental Therapy, Wroclaw Medical University, Wroclaw, Poland
    • Corresponding Author InformationReprint requests: Agnieszka Chrobak, Ph.D., Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50-137 Wrocław, Poland (FAX: 48-71-3752608).
    • ,
  • Urszula Sieradzka, M.D.

      Affiliations

    • Second Department of Gynaecology, Obstetrics and Neonatology, Wroclaw Medical University, Wroclaw, Poland
    • ,
  • Rafał Sozański, M.D.

      Affiliations

    • First Department Of Obstetrics and Gynecology, Wroclaw Medical University, Wroclaw, Poland
    • ,
  • Anna Chełmońska-Soyta, Ph.D.

      Affiliations

    • Laboratory of Reproductive Immunology, Institute of Immunology and Experimental Therapy, Wroclaw Medical University, Wroclaw, Poland
    • ,
  • Marian Gabryś, Ph.D., M.D.

      Affiliations

    • First Department Of Obstetrics and Gynecology, Wroclaw Medical University, Wroclaw, Poland
    • ,
  • Małgorzata Jerzak, Ph.D., M.D.

      Affiliations

    • Department of Gynecology, Military Institute of Medicine, Warsaw, Poland

Received 27 July 2004 ,Revised 21 August 2008 ,Accepted 4 September 2008.

References 

  1. Vinatier D, Cosson M, Dufour P. Is endometriosis an endometrial disease?. Eur J Obstet Gynecol Reprod Biol. 2000;91:113–125
  2. Watanabe H, Kanzaki H, Narukawa S, Inoue T, Katsuragawa H, Kaneko Y, et al. Bcl-2 and Fas expression in eutopic and ectopic human endometrium during the menstrual cycle in relation to endometrial cell apoptosis. Am J Obstet Gynecol. 1997;176:360–368
  3. Gebel HM, Braun DP, Tambur A, Frame D, Rana N, Dmowski WP. Spontaneous apoptosis of endometrial tissue is impaired in women with endometriosis. Fertil Steril. 1998;69:1042–1047
  4. Dmowski WP, Ding J, Shen J, Rana N, Fernandez BB, Braun DP. Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis. Hum Reprod. 2001;16:1802–1808
  5. Otsuki Y. Apoptosis in human endometrium: apoptotic detection methods and signaling. Med Electron Microsc. 2001;34:166–173
  6. Yamashita H, Otsuki Y, Matsumoto K, Ueki K, Ueki M. Fas ligand, Fas antigen and Bcl-2 expression in human endometrium during the menstrual cycle. Mol Hum Reprod. 1999;5:358–364
  7. Tabibzadeh S, Kong QF, Satyaswaroop PG, Zupi E, Marconi D, Romanin C, et al. Distinct regional and menstrual cycle dependent distribution of apoptosis in human endometrium. Potential regulatory role of T cells and TNF-α. Endocrinol J. 1994;2:87–95
  8. Vaskivuo TE, Stenback F, Karhumaa P, Risteli J, Dunkel L, Tapanainen JS. Apoptosis and apoptosis-related proteins in human endometrium. Mol Cell Endocrinol. 2000;165:75–83
  9. Kokawa K, Shikone T, Nakano R. Apoptosis in the human uterine endometrium during the menstrual cycle. J Clin Endocrinol Metab. 1996;81:4144–4147
  10. Dahmoun M, Boman K, Cajander S, Westin P, Backstrom T. Apoptosis, proliferation, and sex hormone receptors in superficial parts of human endometrium at the end of the secretory phase. J Clin Endocrinol Metab. 1999;84:1737–1743
  11. Wu Y, Shi X, Guo SW. The knockdown of progesterone receptor isoform B (PR-B) promotes proliferation in immortalized endometrial stromal cells. Fertil Steril. 2008;90:1320–1323
  12. Tabibzadeh S, Satyaswaroop PG, von Wolff M, Strowitzki T. Regulation of TNF-alpha mRNA expression in endometrial cells by TNF-alpha and by oestrogen withdrawal. Mol Hum Reprod. 1999;5:1141–1149
  13. Kharfi A, Labelle Y, Mailloux J, Akoum A. Deficient expression of tumor necrosis factor receptor type 2 in the endometrium of women with endometriosis. Am J Reprod Immunol. 2003;50:33–40
  14. Jones RK, Bulmer JN, Searle RF. Immunohistochemical characterization of proliferation, oestrogen receptor and progesterone receptor expression in endometriosis: comparison of eutopic and ectopic endometrium with normal cycling endometrium. Hum Reprod. 1995;10:3272–3279
  15. Kolesnick RN, Haimovitz-Friedman A, Fuks Z. The sphingomyelin signal transduction pathway mediates apoptosis for tumor necrosis factor, Fas, and ionizing radiation. Biochem Cell Biol. 1994;72:471–474
  16. Rath PC, Aggarwal BB. TNF-induced signaling in apoptosis. J Clin Immunol. 1999;19:350–364
  17. Rother J, van Echten G, Schwarzmann G, Sandhoff K. Biosynthesis of sphingolipids: dihydroceramide and not sphinganine is desaturated by cultured cells. Biochem Biophys Res Commun. 1992;189:14–20
  18. Gaur U, Aggarwal BB. Regulation of proliferation, survival and apoptosis by members of the TNF superfamily. Biochem Pharmacol. 2003;66:1403–1408
  19. Ohta H, Yatomi Y, Sweeney EA, Hakomori S, Igarashi Y. A possible role of sphingosine in induction of apoptosis by tumor necrosis factor-alpha in human neutrophils. FEBS Lett. 1994;355:267–270
  20. Kosaka K, Fujiwara H, Tatsumi K, Yoshioka S, Higuchi T, Sato Y, et al. Human peripheral blood mononuclear cells enhance cell-cell interaction between human endometrial epithelial cells and BeWo-cell spheroids. Hum Reprod. 2003;18:19–25
  21. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods. 1986;89:271–277
  22. Gong J, Traganos F, Darzynkiewicz Z. A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry. Anal Biochem. 1994;218:314–319
  23. Tabibzadeh S. Signals and molecular pathways involved in apoptosis, with special emphasis on human endometrium. Hum Reprod Update. 1995;1:303–323
  24. Sakakura C, Sweeney EA, Shirahama T, Hakomori S, Igarashi Y. Suppression of bcl-2 gene expression by sphingosine in the apoptosis of human leukemic HL-60 cells during phorbol ester-induced terminal differentiation. FEBS Lett. 1996;379:177–180
  25. Meresman GF, Vighi S, Buquet RA, Contreras-Ortiz O, Tesone M, Rumi LS. Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis. Fertil Steril. 2000;74:760–66
  26. Braun DP, Ding J, Shaheen F, Willey JC, Rana N, Dmowski WP. Quantitative expression of apoptosis-regulating genes in endometrium from women with and without endometriosis. Fertil Steril. 2007;87:263–268
  27. Siskind LJ, Feinstein L, Yu T, Davis JS, Jones D, Choi J, et al. Anti-apoptotic Bcl-2 Family Proteins Disassemble Ceramide Channels. J Biol Chem. 2008;283:6622–6630
  28. Brás A, Albar JP, Leonardo E, de Buitrago GG, Martínez-A C. Ceramide-induced cell death is independent of the Fas/Fas ligand pathway and is prevented by Nur77 overexpression in A20 B cells. Cell Death Differ. 2000;7:262–271
  29. Strelow A, Bernardo K, Adam-Klages S, Linke T, Sandhoff K, Kronke M, et al. Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death. J Exp Med. 2000;192(5):601–612
  30. Alessenko AV. Functions of sphingosine in cell proliferation and death. Biochemistry (Mosc). 1998;63(1):62–68
  31. Balaban N, Moni J, Shannon M, Dang L, Murphy E, Goldkorn T. The effect of ionizing radiation on signal transduction: antibodies to EGF receptor sensitize A431 cells to radiation. Biochim Biophys Acta. 1996;1314(1-2):147–156

 A.C. has nothing to disclose. U.S. has nothing to disclose. R.S. has nothing to disclose. A.C.-S. has nothing to disclose. M.G. has nothing to disclose. M.J. has nothing to disclose.

 Supported by grant No. 2 P05E 176 22 and “POL-POSTDOC II” No. PBZ/MEiN/01/2006/23 from the Ministry of Science and Higher Education. Dr. Chrobak was the prize-winner of The Foundation for Polish Science for the most promising young research workers 2005 and 2006.

PII: S0015-0282(08)03945-9

doi: 10.1016/j.fertnstert.2008.09.035

Fertility and Sterility
Volume 92, Issue 6 , Pages 1834-1843 , December 2009

Article Tools