Fertility and Sterility
Volume 91, Issue 4, Supplement , Pages 1568-1570 , April 2009

Clinical features and reproductive outcomes for embryos undergoing dual freeze-thaw sequences followed by blastocyst transfer: critique of 14 consecutive cases in IVF

  • Eric Scott Sills, M.D.

      Affiliations

    • Sims International Fertility Clinic/The Sims Institute, Dublin, Ireland
    • Corresponding Author InformationReprint requests: E. Scott Sills, M.D., Sims International Fertility Clinic/The Sims Institute, Rosemount Hall, Dundrum Road, Dundrum, Dublin 14, Ireland (FAX: +353-1-296-8512).
    • ,
  • Gráinne U. Murray, B.Sc.

      Affiliations

    • Sims International Fertility Clinic/The Sims Institute, Dublin, Ireland
    • ,
  • Marc G. Genton, Ph.D.

      Affiliations

    • Department of Econometrics, University of Geneva, Geneva, Switzerland
    • ,
  • David J. Walsh, M.D., M.R.C.O.G.

      Affiliations

    • Sims International Fertility Clinic/The Sims Institute, Dublin, Ireland
    • ,
  • Graham D. Coull, B.Sc.

      Affiliations

    • Sims International Fertility Clinic/The Sims Institute, Dublin, Ireland
    • ,
  • Anthony P.H. Walsh, M.D., M.R.C.O.G., M.R.C.P.I.

      Affiliations

    • Sims International Fertility Clinic/The Sims Institute, Dublin, Ireland

Received 5 September 2008 ,Revised 25 September 2008 ,Accepted 25 September 2008.

References 

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  2. Trounson A, Mohr L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature. 1983;305(5936):707–709
  3. Andersen AN, Goossens V, Ferraretti AP, Bhattacharya S, Felberbaum R, de Mouzon J, et al. Assisted reproductive technology in Europe, 2004: results generated from European registers by ESHRE. Hum Reprod. 2008;23:756–771
  4. Sills ES, Walsh DJ, Walsh APH. Results from the advanced reproductive technologies: fresh vs. frozen?. Ir Med J. 2008;101:288
  5. Sills ES, Schattman GL, Veeck LL, Liu HC, Prasad M, Rosenwaks Z. Characteristics of consecutive in vitro fertilization cycles among patients treated with follicle-stimulating hormone (FSH) and human menopausal gonadotropin versus FSH alone. Fertil Steril. 1998;69:831–835
  6. Sills ES, Drews CD, Perloe M, Kaplan CR, Tucker MJ. Periovulatory serum human chorionic gonadotropin (hCG) concentrations following subcutaneous and intramuscular nonrecombinant hCG use during ovulation induction: a prospective, randomized trial. Fertil Steril. 2001;76:397–399
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  8. Damario MA, Hammitt DG, Galanits TM, Session DR, Dumesic DA. Pronuclear stage cryopreservation after intracytoplasmic sperm injection and conventional IVF: implications for timing of the freeze. Fertil Steril. 1999;72:1049–1054
  9. Pantos K, Stefanidis K, Pappas K, Kokkinopoulos P, Petroutsou K, Kokkali G, et al. Cryopreservation of embryos, blastocysts, and pregnancy rates of blastocysts derived from frozen-thawed embryos and frozen-thawed blastocysts. J Assist Reprod Genet. 2001;18:579–582
  10. Kaufman RA, Menezo Y, Hazout A, Nicollet B, DuMont M, Servy EJ. Cocultured blastocyst cryopreservation: experience of more than 500 transfer cycles. Fertil Steril. 1995;64:1125–1129
  11. Smith LK, Roots EH, Dorsett MJ. Live birth of a normal healthy baby after a frozen embryo transfer with blastocysts that were frozen and thawed twice. Fertil Steril. 2005;83:198–200
  12. Baker A, Check JH, Lurie D, Hourani C, Hoover LM. Pregnancy achieved with pronuclear-stage embryos that were cryopreserved and thawed twice: a case report. J Assist Reprod Genet. 1996;13:713–715
  13. Yokota Y, Yokota H, Yokota M, Sato S, Araki Y. Birth of healthy twins from in vitro development of human refrozen embryos. Fertil Steril. 2001;76:1063–1065
  14. Farhat M, Zentner B, Lossos F, Bdolah Y, Holtzer H, Hurwitz A. Successful pregnancy following replacement of embryos previously refrozen at blastocyst stage: case report. Hum Reprod. 2001;16:337–339
  15. Timeus F, Crescenzio N, Saracco P, Doria A, Fazio L, Albiani R, et al. Recovery of cord blood hematopoietic progenitors after successive freezing and thawing procedures. Haematologica. 2003;88:74–79
  16. Gunetti M, Ferrero I, Rustichelli D, Berger M, Gammaitoni L, Timeus F, et al. Refreezing of cord blood hematopoietic stem cells for allogenic transplantation: in vitro and in vivo validation of a clinical phase I/II protocol in European and Italian Good Manufacturing Practice conditions. Exp Hematol. 2008;36:235–243
  17. Estes SJ, Laky DC, Hoover LM, Smith SE, Schinfeld JS, Somkuti SG. Successful pregnancy resulting from cryopreserved pronuclear and cleaved embryos thawed and cultured to blastocysts, refrozen and transferred. A case report. J Reprod Med. 2003;48:46–48
  18. Veeck LL, Amundson CH, Brothman LJ, DeScisciolo C, Maloney MK, Muasher SJ, et al. Significantly enhanced pregnancy rates per cycle through cryopreservation and thaw of pronuclear stage oocytes. Fertil Steril. 1993;59:1202–1207
  19. Horne G, Critchlow JD, Newman MC, Edozien L, Matson PL, Lieberman BA. A prospective evaluation of cryopreservation strategies in a two-embryo transfer programme. Hum Reprod. 1997;12:542–547
  20. Senn A, Vozzi C, Chanson A, DeGrandi P, Germond M. Prospective randomized study of two cryopreservation policies avoiding embryo selection: the pronucleate stage leads to a higher cumulative delivery rate than the early cleavage stage. Fertil Steril. 2002;74:946–952
  21. Karaki RZ, Samarraie SS, Younis NA, Lahloub TM, Ibrahim MH. Blastocyst culture and transfer: a step toward improved in vitro fertilization outcome. Fertil Steril. 2002;77:114–118

 E.S.S. has nothing to disclose. G.U.M. has nothing to disclose. M.G.G. has nothing to disclose. D.J.W. has nothing to disclose. G.D.C. has nothing to disclose. A.P.H.W. has nothing to disclose.

PII: S0015-0282(08)04100-9

doi: 10.1016/j.fertnstert.2008.09.075

Fertility and Sterility
Volume 91, Issue 4, Supplement , Pages 1568-1570 , April 2009

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