A birth from the transfer of a single vitrified-warmed blastocyst using intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient

Kyono, Koichi; Nakajo, Yukiko; Nishinaka, Chikako; Hattori, Hiromitsu; Kyoya, Toshihiko; Ishikawa, Takayuki; Abe, Hiroyuki; Araki, Yasuhisa

doi:10.1016/j.fertnstert.2008.10.002

« Previous Next »Fertility and Sterility
Volume 91, Issue 3 , Pages 931.e7-931.e11, March 2009

A birth from the transfer of a single vitrified-warmed blastocyst using intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient

Koichi Kyono, M.D.
- Kyono ART Clinic, Mitsui-Seimei, Honcho, Aobaku, Sendai, Japan
- Reprint requests: Dr. Koichi Kyono, Kyono ART Clinic, Mitsui-Seimei, Sendai Honcho Bld, 3F, 1-1-1, Honcho, Aobaku, Sendai 980-0014, Japan (FAX: 81-22-722-8840).
Yukiko Nakajo, B.S.
- Kyono ART Clinic, Mitsui-Seimei, Honcho, Aobaku, Sendai, Japan
Chikako Nishinaka, B.S.
- Kyono ART Clinic, Mitsui-Seimei, Honcho, Aobaku, Sendai, Japan
Hiromitsu Hattori, B.S.
- Kyono ART Clinic, Mitsui-Seimei, Honcho, Aobaku, Sendai, Japan
Toshihiko Kyoya, B.S.
- Kyono ART Clinic, Mitsui-Seimei, Honcho, Aobaku, Sendai, Japan
Takayuki Ishikawa, Ph.D.
- Kyono ART Clinic, Mitsui-Seimei, Honcho, Aobaku, Sendai, Japan
Hiroyuki Abe, Ph.D.
- Graduate School of Science and Engineering, Yamagata University, Jyounan, Yonezawa, Yamagata, Japan
Yasuhisa Araki, Ph.D.
- Institute for ARMT, Ishii, Fujimi, Setagun, Gunma, Japan

Received 29 July 2008; received in revised form 27 September 2008; accepted 3 October 2008. published online 01 December 2008.

Objective

To present the effectiveness of diagnostic heterologous intracytoplasmic sperm injection (ICSI), mouse oocyte activation test (MOAT), and ICSI combined with assisted oocyte activation (AOA) in a globozoospermic patient.

Design

A case report.

Setting

A private IVF center, Japan.

Patient(s)

A patient with globozoospermia.

Intervention(s)

MOAT in a mouse and ICSI combined with AOA in a human.

Main Outcome Measure(s)

Ultrastructure, MOAT, fertilization, and pregnancy.

Result(s)

The transmission electron micrographs showed 100% round-headed spermatozoa lacking an acrosome. MOAT showed that the fertilization rate was 68.4% (13/19) when AOA was used but 0% (0/19) when AOA was not used. After the diagnosis of globozoospermia and sperm-related activation deficiency, 17 human mature oocytes were activated with calcium ionophore after ICSI was performed. The fertilization rate was 88.2% (15/17), and 11 blastocysts were cryopreserved using the vitrification method to prevent severe ovarian hyperstimulation syndrome. A single vitrified-warmed blastocyst was transferred. A gestational sac with fetal heart movements was recognized, and a healthy boy weighing 3180 g was born at 40 weeks of gestation by cesarean section without any congenital abnormality.

Conclusion(s)

MOAT allows discrimination between sperm- and oocyte-related fertilization failures and shows the effectiveness of AOA.

Key Words: Globozoospermia, calcium ionophore A23187, strontium chloride (SrCl₂), ICSI, diagnostic heterologous ICSI, assisted oocyte activation (AOA), round-headed spermatozoa, lack of an acrosome

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K.K. has nothing to disclose. Y.N. has nothing to disclose. C.N. has nothing to disclose. H.H. has nothing to disclose. T.K. has nothing to disclose. T.I. has nothing to disclose. H.A. has nothing to disclose. Y.A.

PII: S0015-0282(08)04131-9

doi:10.1016/j.fertnstert.2008.10.002

« Previous Next »Fertility and Sterility
Volume 91, Issue 3 , Pages 931.e7-931.e11, March 2009

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