Fertility and Sterility
Volume 93, Issue 3 , Pages 774-782.e1 , February 2010

Embryologic outcome and secretome profile of implanted blastocysts obtained after coculture in human endometrial epithelial cells versus the sequential system

  • Francisco Dominguez, Ph.D.

      Affiliations

    • Fundación IVI, Instituto Universitario IVI, University of Valencia, Valencia, Spain
    • Corresponding Author InformationReprint requests: Dr. Francisco Dominguez, Fundación Instituto Valenciano de Infertilidad (FIVI), C/Guadassuar 1 bajo, 46015—Valencia, Spain (FAX: +34 96 3455512).
    • ,
  • Blanca Gadea, Ph.D.

      Affiliations

    • Fundación IVI, Instituto Universitario IVI, University of Valencia, Valencia, Spain
    • ,
  • Amparo Mercader, Ph.D.

      Affiliations

    • Fundación IVI, Instituto Universitario IVI, University of Valencia, Valencia, Spain
    • ,
  • Francisco J. Esteban, Ph.D.

      Affiliations

    • Department of Experimental Biology, University of Jaén, Jaén, Spain
    • ,
  • Antonio Pellicer, M.D., Ph.D.

      Affiliations

    • Fundación IVI, Instituto Universitario IVI, University of Valencia, Valencia, Spain
    • Department of Pediatrics, Obstetrics, and Gynecology, Valencia University School of Medicine, Valencia, Spain
    • ,
  • Carlos Simón, M.D., Ph.D.

      Affiliations

    • Fundación IVI, Instituto Universitario IVI, University of Valencia, Valencia, Spain
    • Department of Pediatrics, Obstetrics, and Gynecology, Valencia University School of Medicine, Valencia, Spain

Received 20 August 2008 ,Revised 30 September 2008 ,Accepted 15 October 2008.

References 

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  3. Simon C, Mercader A, Garcia-Velasco J, Nikas G, Moreno C, Remohi J, et al. Coculture of human embryos with autologous human endometrial epithelial cells in patients with implantation failure. J Clin Endocrinol Metab. 1999;84:2638–2646
  4. Mercader A, Garcia-Velasco JA, Escudero E, Remohi J, Pellicer A, Simon C. Clinical experience and perinatal outcome of blastocyst transfer after coculture of human embryos with human endometrial epithelial cells: a 5-year follow-up study. Fertil Steril. 2003;80:1162–1168
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  7. Simón C, Mercader A, Garcia-Velasco J, Nikas G, Moreno C, Remohí J, et al. Coculture of human embryos with autologous human endometrial epithelial cells in patients with implantation failure. J Clin Endocrinol Metab. 1999;84:2638–2646
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  13. Mercader A, Valbuena D, Simón C. Human embryo culture. Methods Enzymol. 2006;420:3–18
  14. Simón C, Piquette G, Frances A, Polan ML. Localization of interleukin-1 type I receptor and interleukin-1 beta in human endometrium throughout the menstrual cycle. J Clin Endocrinol Metab. 1993;77:549–555
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  22. Von Wolff M, Thaler CJ, Zepf C, Becker V, Beier HM, Strowitzki T. Endometrial expression and secretion of interleukin-6 throughout the menstrual cycle. Gynecol Endocrinol. 2002;16:121–129
  23. Sánchez-Cuenca J, Martín JC, Pellicer A, Simón C. Cytokine pleiotropy and redundancy—gp130 cytokines in human implantation. Immunol Today. 1999;20:57–59
  24. Dunglison GF, Barlow DH, Sargent IL. Leukaemia inhibitory factor significantly enhances the blastocyst formation rates of human embryos cultured in serum-free medium. Hum Reprod. 1996;11:191–196
  25. De los Santos MJ, Mercader A, Francés A, Portolés E, Remohí J, Pellicer A, et al. Role of endometrial factors in regulating secretion of components of the immunoreactive human embryonic interleukin-1 system during embryonic development. Biol Reprod. 1996;54:563–574
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 F.D. has nothing to disclose. B.G. has nothing to disclose. A.M. has nothing to disclose. F.E. has nothing to disclose. A.P. has nothing to disclose. C.S. has nothing to disclose.

 The first two authors contributed equally to this work.

PII: S0015-0282(08)04200-3

doi: 10.1016/j.fertnstert.2008.10.019

Fertility and Sterility
Volume 93, Issue 3 , Pages 774-782.e1 , February 2010

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