Fertility and Sterility
Volume 93, Issue 3 , Pages 976-985 , February 2010

High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells

  • Ji Yeon Lee, M.Sc.

      Affiliations

    • Fertility Center, CHA General Hospital, CHA Research Institute, Pochon CHA University, Seoul, South Korea
    • ,
  • Jeoung Eun Lee, M.Sc.

      Affiliations

    • CHA Stem Cell Institute, Pochon CHA University, Seoul, South Korea
    • ,
  • Dong Ku Kim, Ph.D.

      Affiliations

    • CHA Stem Cell Institute, Pochon CHA University, Seoul, South Korea
    • ,
  • Tae Ki Yoon, M.D.

      Affiliations

    • Fertility Center, CHA General Hospital, CHA Research Institute, Pochon CHA University, Seoul, South Korea
    • ,
  • Hyung Min Chung, Ph.D.

      Affiliations

    • Fertility Center, CHA General Hospital, CHA Research Institute, Pochon CHA University, Seoul, South Korea
    • CHA Stem Cell Institute, Pochon CHA University, Seoul, South Korea
    • ,
  • Dong Ryul Lee, Ph.D.

      Affiliations

    • Fertility Center, CHA General Hospital, CHA Research Institute, Pochon CHA University, Seoul, South Korea
    • CHA Stem Cell Institute, Pochon CHA University, Seoul, South Korea
    • Corresponding Author InformationReprint requests: Dong Ryul Lee, Ph.D., Fertility Center, CHA General Hospital, CHA Research Institute, Pochon CHA University, 606-5 Yeoksam-dong, Gangnam-gu, Seoul 135–081, South Korea (FAX: 82-2-501-8704).

Received 27 August 2008 ,Revised 29 September 2008 ,Accepted 7 October 2008.

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 J.Y.L. has nothing to disclose. J.E.L. has nothing to disclose. D.K.K. has nothing to disclose. T.K.Y. has nothing to disclose. H.M.C. has nothing to disclose. D.R.L. has nothing to disclose.

 Supported by a grant (SC1140) from the Stem Cell Research Center, funded by the Ministry of Education, Science and Technology, Korea.

PII: S0015-0282(08)04202-7

doi: 10.1016/j.fertnstert.2008.10.017

Fertility and Sterility
Volume 93, Issue 3 , Pages 976-985 , February 2010

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