Fertility and Sterility
Volume 93, Issue 3 , Pages 807-813 , February 2010

Birefringence characteristics in sperm heads allow for the selection of reacted spermatozoa for intracytoplasmic sperm injection

  • Luca Gianaroli, M.D.

      Affiliations

    • Reproductive Medicine Unit, Italian Society for the Study of Reproductive Medicine, Bologna, Italy
    • Corresponding Author InformationReprint requests: Luca Gianaroli, M.D., Reproductive Medicine Unit, Italian Society for the Study of Reproductive Medicine, Via Mazzini 12, 40128 Bologna, Italy (FAX: 39-051-302933).
    • ,
  • M. Cristina Magli, M.Sc.

      Affiliations

    • Reproductive Medicine Unit, Italian Society for the Study of Reproductive Medicine, Bologna, Italy
    • ,
  • Anna P. Ferraretti, M.D.

      Affiliations

    • Reproductive Medicine Unit, Italian Society for the Study of Reproductive Medicine, Bologna, Italy
    • ,
  • Andor Crippa, Ph.D.

      Affiliations

    • Reproductive Medicine Unit, Italian Society for the Study of Reproductive Medicine, Bologna, Italy
    • ,
  • Michela Lappi, B.Sc.

      Affiliations

    • Reproductive Medicine Unit, Italian Society for the Study of Reproductive Medicine, Bologna, Italy
    • ,
  • Serena Capitani, Ph.D.

      Affiliations

    • Interdipartimentale Centre for Male Infertility, Unit of Applied Biology, Department of Surgery Policlinico, “S. Maria alle Scotte,” Siena, Italy
    • ,
  • Baccio Baccetti, M.D.

      Affiliations

    • Interdipartimentale Centre for Male Infertility, Unit of Applied Biology, Department of Surgery Policlinico, “S. Maria alle Scotte,” Siena, Italy

Received 8 June 2008 ,Revised 6 October 2008 ,Accepted 17 October 2008.

References 

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  2. Bernardini L, Gianaroli L, Fortini D, Conte N, Magli MC, Cavani S, et al. Frequency of hyper, hypohaploidy and diploidy in ejaculate, epididymal and testicular germ cells of infertile patients. Hum Reprod. 2000;15:2165–2172
  3. Bernardini L, Costa M, Bottazzi C, Gianaroli L, Magli MC, Venturini PL, et al. Sperm aneuploidy and recurrent pregnancy loss. Reprod Biomed Online. 2004;9:312–320
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  5. Gianaroli L, Magli MC, Cavallini G, Crippa A, Nadalini M, Bernardini L, et al. Frequency of aneuploidy in spermatozoa from patients with extremely severe male factor infertility. Hum Reprod. 2005;20:2140–2152
  6. Gianaroli L, Magli MC, Collodel G, Moretti E, Ferraretti AP, Baccetti B. Sperm head's birefringence: a new criterion for sperm selection. Fertil Steril. 2008;90:104–112
  7. Baccetti B. Microscopical advances in assisted reproduction. J Submicrosc Cytol Pathol. 2004;36:333–339
  8. Mansour RT, Serour MG, Abbas AM, Kamal A, Tawab NA, Aboulghar MA, et al. The impact of spermatozoa preincubation time and spontaneous acrosome reaction in intracytoplasmic sperm injection: a controlled randomized study. Fertil Steril. 2008;90:584–591
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  10. Sathananthan AH, Szell A, Ng SC, Kausche A, Lacham-Kaplan O, Trounson A. Is the acrosome reaction a prerequisite for sperm incorporation after intracytoplasmic sperm injection (ICSI)?. Reprod Fertil Dev. 1997;9:703–710
  11. Katayama M, Koshida M, Miyake M. Fate of the acrosome in ooplasm in pigs after IVF and ICSI. Hum Reprod. 2002;17:2657–2664
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  17. Magli MC, Gianaroli L, Ferraretti AP, Lappi M, Ruberti A, Farfalli V. Embryo morphology and development is dependent on the chromosomal complement. Fertil Steril. 2007;87:534–541
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  22. Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Yagoda A, Lederman H, et al. Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril. 2003;80:1413–1419
  23. Berkovitz A, Eltes F, Lederman H, Peer S, Ellenborgen A, Feldberg B, et al. How to improve IVF-ICSI outcome by sperm selection. Reprod Biomed Online. 2006;12:634–638
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 L.G. has nothing to disclose. M.C.M. has nothing to disclose. A.P.F. has nothing to disclose. A.C. has nothing to disclose. M.L. has nothing to disclose. S.C. has nothing to disclose. B.B. has nothing to disclose.

PII: S0015-0282(08)04280-5

doi: 10.1016/j.fertnstert.2008.10.024

Fertility and Sterility
Volume 93, Issue 3 , Pages 807-813 , February 2010

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