Fertility and Sterility
Volume 93, Issue 2 , Pages 364-373 , 15 January 2010

Preimplantation genetic screening for aneuploidy of embryos after in vitro fertilization in women aged at least 35 years: a prospective randomized trial

Presented in part at the 63rd Annual Meeting of the American Society for Reproductive Medicine, Washington, DC, October 15–17, 2007.

  • Sophie Debrock, Ph.D.

      Affiliations

    • Leuven University Fertility Center, Leuven, Belgium
    • ,
  • Cindy Melotte, M.Sc.

      Affiliations

    • Center for Human Genetics, Leuven, Belgium
    • ,
  • Carl Spiessens, Ph.D.

      Affiliations

    • Leuven University Fertility Center, Leuven, Belgium
    • ,
  • Karen Peeraer, M.D.

      Affiliations

    • Leuven University Fertility Center, Leuven, Belgium
    • ,
  • Evelyne Vanneste, M.Sc.

      Affiliations

    • Leuven University Fertility Center, Leuven, Belgium
    • Center for Human Genetics, Leuven, Belgium
    • ,
  • Luc Meeuwis, M.D.

      Affiliations

    • Leuven University Fertility Center, Leuven, Belgium
    • ,
  • Christel Meuleman, M.D.

      Affiliations

    • Leuven University Fertility Center, Leuven, Belgium
    • ,
  • Jean-Pierre Frijns, M.D., Ph.D.

      Affiliations

    • Center for Human Genetics, Leuven, Belgium
    • ,
  • Joris R. Vermeesch, Ph.D.

      Affiliations

    • Center for Human Genetics, Leuven, Belgium
    • ,
  • Thomas M. D'Hooghe, M.D., Ph.D.

      Affiliations

    • Center for Human Genetics, Leuven, Belgium
    • Corresponding Author InformationReprint requests: Thomas M. D'Hooghe, M.D., Ph.D., Center for Human Genetics, UZ Leuven, Herestraat 49, B-3000 Leuven, Belgium (FAX: 32-16-344368).

Received 23 May 2008 ,Revised 30 October 2008 ,Accepted 30 October 2008.

References 

  1. Shahine LK, Cedars MI. Preimplantation genetic diagnosis does not increase pregnancy rates in patients at risk for aneuploidy. Fertil Steril. 2006;85:51–56
  2. Twisk M, Mastenbroek S, van Wely M, Heineman MJ, Van der Veen F, Repping S. Preimplantation genetic screening for abnormal number of chromosomes (aneuploidies) in in vitro fertilisation or intracytoplasmic sperm injection. Cochrane Database Syst Rev. 2006;1:CD005291
  3. Staessen C, Platteau P, Van Assche E, Michiels A, Tournaye H, Camus M, et al. Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomized controlled trial. Hum Reprod. 2004;19:2849–2858
  4. Mastenbroek S, Twisk M, van Echten-Arends J, Sikkema-Raddatz B, Korevaar JC, Verhoeve HR, et al. In vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;357:9–17
  5. Hardarson T, Hanson C, Lundin K, Hillensjö T, Nilsson L, Stevic J, et al. Preimplantation genetic screening in women of advanced maternal age caused a decrease in clinical pregnancy rate: a randomized controlled trial. Hum Reprod. 2008;23:2806–2812
  6. Schoolcraft WB, Katz-Jaffe MG, Stevens J, Rawlins M, Munné S. Preimplantation aneuploidy testing for infertile patients of advanced maternal age: a randomized prospective trial. Fertil Steril. Published online 8 August 2008 [Epub ahead of print].
  7. Van Landuyt L, De Vos A, Joris H, Verheyen G, Devroey P, Van Steirteghem A. Blastocyst formation in in vitro fertilization versus intracytoplasmic sperm injection cycles: influence of the fertilization procedure. Fertil Steril. 2005;83:1397–1403
  8. Koninklijk Besluit tot wijziging van het KB van 25 April 2002 betreffende de vastselling en de vereffening van het budget van financiële middelen voor de ziekenhuizen. Modaliteiten voor de regeling inzake medisch geassisteerde voortplanting. Belgisch Staatsblad. 2003;16:32133–32157
  9. Melotte C, Debrock S, D'Hooghe T, Fryns JP, Vermeesch JR. Preimplantation genetic diagnosis for an insertional translocation carrier. Hum Reprod. 2004;19:2777–2783
  10. Munné S, Weier HU. Simultaneous enumeration of chromosomes 13, 18, 21, X, and Y in interphase cells for preimplantation genetic diagnosis of aneuploidy. Cytogenet Cell Genet. 1996;75:263–270
  11. Staessen C, Platteau P, Van Assche E, Michiels A, Tournaye H, Camus M, et al. Reply to comments on Staessen, et al. (2004). [letter] Hum Reprod. 2005;20:2364–2365
  12. Gianaroli L, Magli C, Ferraretti AP, Munné S. Preimplantation diagnosis for aneuploidies in patients undergoing in vitro fertilization with a poor prognosis: identification of the categories for which it should be proposed. Fertil Steril. 1999;72:837–844
  13. Munné S, Magli C, Cohen J, Morton P, Sadowy S, Gianaroli L, et al. Positive outcome after preimplantation diagnosis of aneuploidy in human embryos. Hum Reprod. 1999;14:2191–2199
  14. Munné S, Sandalinas M, Escudero T, Velilla E, Walmsley R, Sadowy S, et al. Improved implantation after preimplantation genetic diagnosis of aneuploidy. RBM Online. 2003;7:91–97
  15. Colls P, Escudero T, Cekleniak N, Sadowy S, Cohen J, Munné S. Increased efficiency of preimplantation genetic diagnosis for infertility using “no result rescue.”. Fertil Steril. 2007;88:53–61
  16. Mastenbroek S, Bossuyt P, Heineman MJ, Repping S, van der Veen F, et al. Comment 1 on Staessen, et al. (2004): design and analysis of a randomized controlled trial studying preimplantation genetic screening. [letter] Hum Reprod. 2005;20:2362–2363
  17. Cohen J, Wells D, Munné S. Removal of 2 cells from cleavage stage embryos is likely to reduce the efficacy of chromosomal tests that are used to enhance implantation rates. Fertil Steril. 2007;87:496–503
  18. Combelles CMH. What are the trade-offs between one-cell and two-cell biopsies of preimplantation embryos? Associate editor's comment on the fast-track article “Diagnostic efficiency, embryonic development and clinical outcome after the biopsy of one or two blastomeres for preimplantation genetic diagnosis” by Goossens, et al. Hum Reprod. 2008;23:493–498
  19. Cieslak-Janzen J, Tur-Kaspa I, Ilkevitch Y, Bernal A, Morris R, Verlinsky Y. Multiple micromanipulations for PGD do not affect embryo development to the blastocyst stage. Fertil Steril. 2006;85:1826–1829
  20. Van de Velde H, De Vos A, Sermon K, Staessen C, De Rycke M, Van Assche E, et al. Embryo implantation after biopsy of one or two cells from cleavage-stage embryos with a view to preimplantation genetic diagnosis. Prenat Diagn. 2000;20:1030–1037
  21. Wilton LJ Comments to Mastenbroek et al. in vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;(357):1769–1770
  22. Goossens V, De Rycke M, De Vos A, Staessen C, Michiels A, Verpoest W, et al. Diagnostic efficiency, embryonic development and clinical outcome after the biopsy of one or two blastomeres for preimplantation genetic diagnosis. Hum Reprod. 2008;23:481–492
  23. Munné S, Cohen J. Comments to Mastenbroek et al. (2007): in vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;357:1769–1770
  24. Wilton L. Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization. Hum Reprod Update. 2005;11:33–41
  25. Le Caignec C, Spits C, Sermon K, De Rycke M, Thienpont B, Debrock S, et al. Single-cell chromosomal imbalances detection by arry CGH. Nucleic Acids Res. 2006;34:e68
  26. Gleicher N, Weghoger A, Barad D. Preimplantation genetic screening: “established” and ready for prime time?. Fertil Steril. 2008;89:780–788

 SD. has nothing to disclose. C.M. has nothing to disclose. C.S. has nothing to disclose. K.P. has nothing to disclose. E.V. has nothing to disclose. L.M. has nothing to disclose. C.M. has nothing to disclose. J.-.P.F. has nothing to disclose. J.R.V. has nothing to disclose. T.M.D. has nothing to disclose.

 Supported by a grant from the Fund for Scientific Research (FWO).

PII: S0015-0282(08)04433-6

doi: 10.1016/j.fertnstert.2008.10.072

Fertility and Sterility
Volume 93, Issue 2 , Pages 364-373 , 15 January 2010

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