Fertility and Sterility
Volume 93, Issue 4 , Pages 1142-1146, 1 March 2010

Testicular spermatozoa have statistically significantly lower DNA damage compared with ejaculated spermatozoa in patients with unsuccessful oral antioxidant treatment

Presented in part at the Annual Meeting of the American Urological Association, Anaheim, California, May 19–24, 2007.

  • Sergey I. Moskovtsev, M.D., Ph.D.

      Affiliations

    • Andrology Laboratory, Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada
    • Corresponding Author InformationReprint requests: S.I. Moskovtsev, M.D., Ph.D., Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Room 6-303, Toronto, Ontario, M5G 1X5 Canada (FAX: 416-586-8589).
    • ,
  • Keith Jarvi, M.D.

      Affiliations

    • Division of Urology, Department of Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada
    • ,
  • J. Brendan M. Mullen, M.D.

      Affiliations

    • Andrology Laboratory, Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada
    • ,
  • Kenneth I. Cadesky, M.D.

      Affiliations

    • LifeQuest Centre for Reproductive Medicine, Toronto, Ontario, Canada
    • ,
  • Thomas Hannam, M.D.

      Affiliations

    • Hannam Fertility Centre, Toronto, Ontario, Canada
    • ,
  • Kirk C. Lo, M.D.

      Affiliations

    • Division of Urology, Department of Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada

Received 26 August 2008; received in revised form 9 October 2008; accepted 2 November 2008. published online 23 December 2008.

Objective

To compare DNA damage in ejaculated and testicular spermatozoa in patients with previously unsuccessful oral antioxidant treatment.

Design

Prospective clinical study.

Setting

University-affiliated teaching hospital.

Patient(s)

Twelve men with persistently high sperm DNA damage.

Intervention(s)

Evaluation of DNA damage of ejaculated and testicular spermatozoa by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay.

Main Outcome Measure(s)

The DNA damage of ejaculated spermatozoa compared with that of testicular spermatozoa, both samples collected on the day of intracytoplasmic sperm injection.

Result(s)

Ejaculated spermatozoa showed a threefold higher DNA damage when compared with testicular samples (39.7% ± 14.8 vs. 13.3% ± 7.3).

Conclusion(s)

Our results indicated that in patients with previously unsuccessful oral antioxidant treatment the retrieved testicular spermatozoa had a lower degree of DNA damage compared with ejaculated sperm collected on the same day.

Key Words: Sperm DNA damage, testicular spermatozoa, DNA fragmentation index, TUNEL

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

 S.I.M. has nothing to disclose. K.J. is a paid consultant for Bayer. J.B.M.M. has nothing to disclose. K.I.C. has nothing to disclose. T.H. has nothing to disclose. K.C.L. is an employee of Bayer Canada.

PII: S0015-0282(08)04551-2

doi:10.1016/j.fertnstert.2008.11.005

Fertility and Sterility
Volume 93, Issue 4 , Pages 1142-1146, 1 March 2010

Article Tools