Fertility and Sterility
Volume 93, Issue 4 , Pages 1147-1156 , 1 March 2010

The effect of repeated freezing and thawing on human sperm DNA fragmentation

  • Laura Kelly Thomson, B.Med.Sc.

      Affiliations

    • Fertility First, Hurstville, Australia
    • Corresponding Author InformationReprint requests: Laura Kelly Thomson, B.Med.Sc., Fertility First, P.O. Box 807, Hurstville NSW 2220, Australia (FAX: +61 2 9570 8664).
    • ,
  • Steven Denis Fleming, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynaecology, Westmead Hospital, University of Sydney, Sydney, Australia
    • ,
  • Katrina Barone, BSc.

      Affiliations

    • Fertility First, Hurstville, Australia
    • ,
  • Julie-Anne Zieschang, M.Med.

      Affiliations

    • Fertility First, Hurstville, Australia
    • ,
  • Anne Melton Clark, MPS, MBCHB, FRCOG, FRANZCOG, CREI

      Affiliations

    • Fertility First, Hurstville, Australia

Received 9 September 2008 ,Revised 29 October 2008 ,Accepted 15 November 2008.

References 

  1. Sherman JK. Synopsis of the use of frozen human semen since 1964: state of the art of human semen banking. Fertil Steril. 1973;24:397–412
  2. Nallella KP, Sharma RK, Allamaneni SS, Aziz N, Agarwal A. Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants. Fertil Steril. 2004;82:913–918
  3. Donnelly ET, Steele EK, McClure N, Lewis SE. Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation. Hum Reprod. 2001;16:1191–1199
  4. de Paula TS, Bertolla RP, Spaine DM, Cunha MA, Schor N, Cedenho AP. Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia. Fertil Steril. 2006;86:597–600
  5. Hammadeh ME, Askari AS, Georg T, Rosenbaum P, Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphologic alteration and membrane integrity of human spermatozoa in fertile and subfertile men. Int J Androl. 1999;22:155–162
  6. Thomson LK, Fleming SD, Schulke L, Barone K, Zieschang J-A, Clark AM. The DNA integrity of cryopreserved spermatozoa separated for use in ART is unaffected by the type of cryoprotectant used but is related to the DNA integrity of the fresh separated preparation. Fertil Steril. 2008 Sept 13;[Epub ahead of print]
  7. Polcz TE, Stronk J, Xiong C, Jones EE, Olive DL, Huszar G. Optimal utilization of cryopreserved human semen for assisted reproduction: recovery and maintenance of sperm motility and viability. J Assist Reprod Genet. 1998;15:504–512
  8. Rofeim O, Brown TA, Gilbert BR. Effects of serial thaw–refreeze cycles on human sperm motility and viability. Fertil Steril. 2001;75:1242–1243
  9. Bandularatne E, Bongso A. Evaluation of human sperm function after repeated freezing and thawing. J Androl. 2002;23:242–249
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  11. Benchaib M, Braun V, Lornage J, Hadj S, Salle B, Lejeune H, et al. Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum Reprod. 2003;18:1023–1028
  12. Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril. 2004;82:378–383
  13. Borini A, Tarozzi N, Bizzaro D, Bonu MA, Fava L, Flamigni C, et al. Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART. Hum Reprod. 2006;21:2876–2881
  14. Duran EH, Morshedi M, Taylor S, Oehninger S. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod. 2002;17:3122–3128
  15. Benchaib M, Lornage J, Mazoyer C, Lejeune H, Salle B, Francois Guerin J. Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril. 2007;87:93–100
  16. Aitken RJ, Krausz C. Oxidative stress, DNA damage and the Y chromosome. Reproduction. 2001;122:497–506
  17. Aitken RJ, De Iuliis GN. Origins and consequences of DNA damage in male germ cells. Reprod Biomed Online. 2007;14:727–733
  18. WHO . WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge, UK: Cambridge University Press; 1999;
  19. Donnelly ET, McClure N, Lewis SE. Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity. Fertil Steril. 2001;76:892–900
  20. Gilmore JA, Liu J, Gao DY, Critser JK. Determination of optimal cryoprotectants and procedures for their addition and removal from human spermatozoa. Hum Reprod. 1997;12:112–118
  21. Wundrich K, Paasch U, Leicht M, Glander H-J. Activation of caspases in human spermatozoa during cryopreservation—an immunoblot study. Cell Tissue Banking. 2006;7:81–90
  22. Paasch U, Sharma RK, Gupta AK, Grunewald S, Mascha EJ, Thomas AJ, et al. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod. 2004;71:1828–1837
  23. Paasch U, Grunewald S, Agarwal A. Glandera H-J. Activation pattern of caspases in human spermatozoa. Fertil Steril. 2004;81(Suppl 1):802–809
  24. Marchetti C, Gallego M-A, Defossez A, Formstecher P, Marchetti P. Staining of human sperm with fluorochrome-labeled inhibitor of caspases to detect activated caspases: correlation with apoptosis and sperm parameters. Hum Reprod. 2004;19:1127–1134
  25. Tremellen K. Oxidative stress and male infertility—a clinical perspective. Hum Reprod Update. 2008;14:243–258
  26. Shekarriz M, DeWire DM, Thomas AJ, Agarwal A. A method of human semen centrifugation to minimize the iatrogenic sperm injuries caused by reactive oxygen species. Eur Urol. 1995;28:31–35
  27. Stevanato J, Bertolla RP, Barradas V, Spaine DM, Cedenho AP, Ortiz V. Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates. Fertil Steril. 2008;90:889–890
  28. Sun JG, Jurisicova A, Casper RF. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod. 1997;56:602–607

 L.K.T. has nothing to disclose. S.D.F. has nothing to disclose. K.B. has nothing to disclose. J.-A.Z. has nothing to disclose. A.M.C. has nothing to disclose.

 Supported and fully funded by Dr. Anne Clark, owner and Medical Director of Fertility First, as part of Miss Thomson's Ph.D. studentship.

PII: S0015-0282(08)04585-8

doi: 10.1016/j.fertnstert.2008.11.023

Fertility and Sterility
Volume 93, Issue 4 , Pages 1147-1156 , 1 March 2010

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