Fertility and Sterility
Volume 93, Issue 5 , Pages 1440-1447, 15 March 2010

15-Epi-lipoxin A4 inhibits the progression of endometriosis in a murine model

  • Qiong-Hua Chen, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Tongji Hospital of Tongji Medical college of Huazhong University of Science & Technology, Wuhan, People's Republic of China
    • Department of Obstetrics and Gynecology, Xiamen First Hospital affiliated by Fujian Medical University, Xiamen, People's Republic of China
    • ,
  • Wei-Dong Zhou, Ph.D.

      Affiliations

    • Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, People's Republic of China
    • ,
  • De-Min Pu, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Tongji Hospital of Tongji Medical college of Huazhong University of Science & Technology, Wuhan, People's Republic of China
    • Corresponding Author InformationReprint requests: De-Min Pu, M.D., Department of Obstetrics and Gynecology, Tongji Hospital of Tongji Medical college of Huazhong University of Science & Technology, Wuhan 430030, P.R. China (FAX: +86-027-83629052).
    • ,
  • Qian-Sheng Huang, Ph.D.

      Affiliations

    • Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, People's Republic of China
    • ,
  • Tian Li, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Tongji Hospital of Tongji Medical college of Huazhong University of Science & Technology, Wuhan, People's Republic of China
    • ,
  • Qing-Xi Chen, Ph.D.

      Affiliations

    • Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, People's Republic of China

Received 7 November 2008; received in revised form 13 January 2009; accepted 15 January 2009. published online 06 March 2009.

Objective

To examine the pro-resolution actions of 15-epi-lipoxin A4 (LXA4) on endometriotic lesions, on the concentrations and activities of matrix metalloproteinases (MMP-2 and MMP-9) in murine endometriosis.

Design

Prospective, vehicle-controlled experimental study.

Setting

Animal research facility.

Animal(s)

BALB/c mice.

Intervention(s)

Endometriosis (EM) was induced in 30 mice. Fifteen of them were administered LXA4 for 24 days (LXA4 group), whereas the other 15 served as a control group (EM group). Another 15 sham-operated mice (sham-operated group) were treated with vehicles.

Main Outcome Measure(s)

The weight of the endometriotic lesions was measured. The concentrations, mRNA, and activities of MMP-2 and MMP-9 were determined by enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, and gelatin zymography, respectively.

Result(s)

Compared with EM group, the weight of the endometriotic lesions was decreased, the concentrations of MMP-2 and MMP-9 dropped, the mRNA levels of MMP-2 and MMP-9 in the peritoneal fluid cells and the endometriotic lesions were reduced, and the activities of MMP-2 and MMP-9 were inhibited in the LXA4 group.

Conclusion(s)

LXA4 may inhibit the progression of endometriosis possibly by lowering the concentrations and the activities of MMP-2 and MMP-9.

Key Words: Endometriosis, Murine model, LXA4, MMP-2, MMP-9

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 Q.-H.C. has nothing to disclose. W.-D.Z. has nothing to disclose. D.-M.P. has nothing to disclose.Q.-S.H. has nothing to disclose. T.L. has nothing to disclose. Q.-X.C. has nothing to disclose.

 Supported by grant 2007-1-43 of the Ministry of Health of Fujian Province, P.R. China, by grant 2009-CXB-49 of the medical innovation project of Fujian Province, P.R. China, by grant 3502 Z 20077047 of the Science & Technology Planning Project of Xiamen City, P.R. China, and by grant 3502 Z 20084006 of the Science & Technology Planning Project of Xiamen City, P R. China.

PII: S0015-0282(09)00143-5

doi:10.1016/j.fertnstert.2009.01.107

Fertility and Sterility
Volume 93, Issue 5 , Pages 1440-1447, 15 March 2010

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