Fertility and Sterility
Volume 93, Issue 5 , Pages 1430-1439 , 15 March 2010

The oocyte spindle is preserved by 1,2-propanediol during slow freezing

Presented in part at the annual meeting of American Society for Reproductive Medicine, Washington, DC, October13–17, 2007.

  • Ching-Chien Chang, Ph.D.

      Affiliations

    • Reproductive Biology Associates, Atlanta, Georgia
    • ,
  • Li-Ying Sung, Ph.D.

      Affiliations

    • Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut
    • Department of Animal Science, University of Connecticut, Storrs, Connecticut
    • ,
  • Chih-Jen Lin, M.Sc.

      Affiliations

    • Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut
    • Department of Animal Science, University of Connecticut, Storrs, Connecticut
    • ,
  • Hilton I. Kort, M.D.

      Affiliations

    • Reproductive Biology Associates, Atlanta, Georgia
    • ,
  • Xiangzhong Yang, Ph.D.

      Affiliations

    • Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut
    • Department of Animal Science, University of Connecticut, Storrs, Connecticut
    • ,
  • X. Cindy Tian, Ph.D.

      Affiliations

    • Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut
    • Department of Animal Science, University of Connecticut, Storrs, Connecticut
    • ,
  • Zsolt Peter Nagy, M.D., Ph.D.

      Affiliations

    • Reproductive Biology Associates, Atlanta, Georgia
    • Corresponding Author InformationReprint requests: Zsolt Peter Nagy, M.D., Ph.D., Reproductive Biology Associates, 1150 Lake Hearn Drive, Suite 600, Atlanta, GA 30342 (FAX: 404-256-1912).

Received 24 October 2008 ,Revised 10 December 2008 ,Accepted 9 January 2009.

References 

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  27. Rienzi L, Martinez F, Ubaldi F, Minasi MG, Iacobelli M, Tesarik J, et al. Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures. Hum Reprod. 2004;19:655–659
  28. Bianchi V, Coticchio G, Fava L, Flamigni C, Borini A. Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration. Hum Reprod. 2005;20:1078–1083
  29. Pickering SJ, Braude PR, Johnson MH, Cant A, Currie J. Transient cooling to RT can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil Steril. 1990;54:102–108
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 C.-C.C. had nothing to disclose. L.-Y.S. had nothing to disclose. C.-J.L. has nothing to disclose. H.I.K. has nothing to disclose. X.Y. had nothing to disclose. X.C.T. had nothing to disclose. Z.P. N. has nothing to disclose.

PII: S0015-0282(09)00144-7

doi: 10.1016/j.fertnstert.2009.01.106

Fertility and Sterility
Volume 93, Issue 5 , Pages 1430-1439 , 15 March 2010

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