First report of the absence of viral load in testicular sperm samples obtained from men with hepatitis C and HIV after washing and their subsequent use

Received 17 December 2008; received in revised form 28 January 2009; accepted 29 January 2009. published online 27 March 2009.

Human immunodeficiency virus and hepatitis C infections are sexually transmitted diseases that require sperm samples to be pretreated to eliminate the viral presence before their safe use in assisted reproduction treatments. In this report we describe our experience with sperm washing protocols applied to sperm cells from testicular biopsies as well as the results obtained in subsequent assisted reproduction treatments on seropositive males that are also azoospermic.

a Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain

b Hospital Universitario Dr. Peset, Valencia, Spain

c Hospital Clínico Universitario, Valencia, Spain

Reprint requests: Dr. Nicolás Garrido, Instituto Universitario IVI, Plaza de la Policía Local, 3, Valencia 46015, Spain (FAX: 3496-3050999).

N.G. has nothing to disclose. M.G.-S. has nothing to disclose. J.M.-J. has nothing to disclose. M.M. has nothing to disclose.

Presented at the 34th annual meeting of the American Society of Andrology, 4-7, April 2009, Philadelphia, Pennsylvania.

PII: S0015-0282(09)00278-7

doi:10.1016/j.fertnstert.2009.01.159

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