Fertility and Sterility
Volume 92, Issue 3 , Pages 1012-1015 , September 2009

First report of the absence of viral load in testicular sperm samples obtained from men with hepatitis C and HIV after washing and their subsequent use

  • Nicolás Garrido, Ph.D.

      Affiliations

    • Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain
    • Corresponding Author InformationReprint requests: Dr. Nicolás Garrido, Instituto Universitario IVI, Plaza de la Policía Local, 3, Valencia 46015, Spain (FAX: 3496-3050999).
    • ,
  • Manuel Gil-Salom, M.D.

      Affiliations

    • Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain
    • Hospital Universitario Dr. Peset, Valencia, Spain
    • ,
  • Jose M. Martínez-Jabaloyas, M.D.

      Affiliations

    • Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain
    • Hospital Clínico Universitario, Valencia, Spain
    • ,
  • Marcos Meseguer, Ph.D.

      Affiliations

    • Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain
    • Hospital Universitario Dr. Peset, Valencia, Spain

Received 17 December 2008 ,Revised 28 January 2009 ,Accepted 29 January 2009.

References 

  1. Centers for Disease Control and Prevention. Sexually transmitted diseases treatment guidelines 2002. MMWR Recomm Rep. 2002;51:1–78
  2. Garrido N, Meseguer M, Bellver J, Remohi J, Simon C, Pellicer A. Report of the results of a 2 year programme of sperm wash and ICSI treatment for human immunodeficiency virus and hepatitis C virus serodiscordant couples. Hum Reprod. 2004;19:2581–2586
  3. Meseguer M, Garrido N, Gimeno C, Remohi J, Simon C, Pellicer A. Comparison of polymerase chain reaction-dependent methods for determining the presence of human immunodeficiency virus and hepatitis C virus in washed sperm. Fertil Steril. 2002;78:1199–1202
  4. Garrido N, Meseguer M, Remohi J, Simon C, Pellicer A. Semen characteristics in human immunodeficiency virus (HIV)- and hepatitis C (HCV)-seropositive males: predictors of the success of viral removal after sperm washing. Hum Reprod. 2005;20:1028–1034
  5. Garrido N, Remohi J, Pellicer A, Meseguer M. The effectiveness of modified sperm washes in severely oligoasthenozoospermic men infected with human immunodeficiency and hepatitis C viruses. Fertil Steril. 2006;86:1544–1546
  6. Garrido N, Meseguer M, Remohi J, Simon C, Pellicer A. Semen characteristics in human immunodeficiency virus (HIV)- and hepatitis C (HCV)-seropositive males: predictors of the success of viral removal after sperm washing. Hum Reprod. 2005;20:1028–1034
  7. Nicopoullos JD, Frodsham LC, Ramsay JW, Almeida PA, Rozis G, Gilling-Smith C. Synchronous sperm retrieval and sperm washing in an intracytoplasmic sperm injection cycle in an azoospermic man who was positive for human immunodeficiency virus. Fertil Steril. 2004;81:670–674
  8. Bujan L, Daudin M, Moinard N, Plante P, Parinaud J, Pasquier C. Azoospermic HIV-1 infected patients wishing to have children: proposed strategy to reduce HIV-1 transmission risk during sperm retrieval and intracytoplasmic sperm injection: Case Report. Hum Reprod. 2007;22:2377–2381
  9. Gil-Salom M, Minguez Y, Rubio C, Remohi J, Pellicer A. Intracytoplasmic testicular sperm injection: an effective treatment for otherwise intractable obstructive azoospermia. J Urol. 1995;154:2074–2077
  10. Gil-Salom M, Romero J, Minguez Y, Rubio C, De los Santos MJ, Remohi J, et al. Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Hum Reprod. 1996;11:1309–1313
  11. Meseguer M, Garrido N, Remohi J, Pellicer A, Simon C, Martinez-Jabaloyas JM, et al. Testicular sperm extraction (TESE) and ICSI in patients with permanent azoospermia after chemotherapy. Hum Reprod. 2003;18:1281–1285
  12. Meseguer M, Garrido N, Martinez-Conejero JA, Simon C, Pellicer A, Remohi J. Role of cholesterol, calcium, and mitochondrial activity in the susceptibility for cryodamage after a cycle of freezing and thawing. Fertil Steril. 2004;81:588–594
  13. Barreto Melo MA, Meseguer M, Bellver J, Remohi J, Pellicer A, Garrido N. Human immunodeficiency type-1 virus (HIV-1) infection in serodiscordant couples (SDCs) does not have impact on embryo quality or intracytoplasmic sperm injection (ICSI) outcome. Fertil Steril. 2008;89:141–150
  14. Speck CE, Coombs RW, Koutsky LA, Zeh J, Ross SO, Hooton TM, et al. Risk factors for HIV-1 shedding in semen. Am J Epidemiol. 1999;150:622–631
  15. Tachet A, Dulioust E, Salmon D, De Almeida M, Rivalland S, Finkielsztejn L, et al. Detection and quantification of HIV-1 in semen: identification of a subpopulation of men at high potential risk of viral sexual transmission. AIDS. 1999;13:823–831

 N.G. has nothing to disclose. M.G.-S. has nothing to disclose. J.M.-J. has nothing to disclose. M.M. has nothing to disclose.

 Presented at the 34th annual meeting of the American Society of Andrology, 4-7, April 2009, Philadelphia, Pennsylvania.

PII: S0015-0282(09)00278-7

doi: 10.1016/j.fertnstert.2009.01.159

Fertility and Sterility
Volume 92, Issue 3 , Pages 1012-1015 , September 2009

Article Tools