Fertility and Sterility
Volume 94, Issue 2 , Pages 477-484, July 2010

A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos

  • Nathan R. Treff, Ph.D.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • Division of Reproductive Endocrinology, Department of Obstetrics Gynecology and Reproductive Science, University of Medicine and Dentistry of New Jersey–Robert Wood Johnson Medical School, New Brunswick, New Jersey
    • Corresponding Author InformationReprint requests: Nathan R. Treff, 111 Madison Ave, Suite 100, Morristown, New Jersey 07960 (FAX: 973-290-8370).
    • ,
  • Jing Su, M.Sc.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • ,
  • Xin Tao, M.Sc.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • ,
  • Kathleen A. Miller, B.Sc.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • ,
  • Brynn Levy, M.Sc.(Med)., Ph.D.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York
    • ,
  • Richard T. Scott Jr., M.D., H.C.L.D.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • Division of Reproductive Endocrinology, Department of Obstetrics Gynecology and Reproductive Science, University of Medicine and Dentistry of New Jersey–Robert Wood Johnson Medical School, New Brunswick, New Jersey

Received 13 February 2009; received in revised form 11 March 2009; accepted 12 March 2009. published online 27 April 2009.

Objective

To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other.

Design

Prospective, randomized, and blinded study.

Setting

Academic center for reproductive medicine.

Patient(s)

Blastomeres were obtained from discarded and transferred embryos from six patients undergoing IVF treatment.

Intervention(s)

None.

Main Outcome Measure(s)

Single lymphocytes from sibling cell lines and blastomeres from sibling day 3 human embryos were evaluated for accurate assignment of relationship using whole genome amplification and single-nucleotide polymorphism microarrays.

Result(s)

Assignment of single-cell relationships was accomplished with 100% accuracy. We also observed complete agreement between the molecular karyotype and DNA fingerprint–based identification of embryos implanted in three clinical IVF cases after multiple embryo transfer.

Conclusion(s)

These data demonstrate the first single-blastomere DNA fingerprinting technology capable of unequivocal discrimination of sibling human embryos.

Key Words: Blastomere, DNA fingerprinting, DNA microarray, single nucleotide polymorphism, whole genome amplification

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 N.T., K.M., and R.S. are supported by grants from EMD Serono, Schering Plough, Ferring Pharmaceuticals, and Barr Pharmaceuticals. The spouse of J.S. is an employee of GE Healthcare. B.L. is a consultant and advisory board member for Affymetrix. X.T. has nothing to disclose.

PII: S0015-0282(09)00637-2

doi:10.1016/j.fertnstert.2009.03.067

Fertility and Sterility
Volume 94, Issue 2 , Pages 477-484, July 2010

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