Fertility and Sterility
Volume 94, Issue 2 , Pages 477-484 , July 2010

A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos

  • Nathan R. Treff, Ph.D.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • Division of Reproductive Endocrinology, Department of Obstetrics Gynecology and Reproductive Science, University of Medicine and Dentistry of New Jersey–Robert Wood Johnson Medical School, New Brunswick, New Jersey
    • Corresponding Author InformationReprint requests: Nathan R. Treff, 111 Madison Ave, Suite 100, Morristown, New Jersey 07960 (FAX: 973-290-8370).
    • ,
  • Jing Su, M.Sc.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • ,
  • Xin Tao, M.Sc.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • ,
  • Kathleen A. Miller, B.Sc.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • ,
  • Brynn Levy, M.Sc.(Med)., Ph.D.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York
    • ,
  • Richard T. Scott Jr., M.D., H.C.L.D.

      Affiliations

    • Reproductive Medicine Associates of New Jersey, Morristown, New Jersey
    • Division of Reproductive Endocrinology, Department of Obstetrics Gynecology and Reproductive Science, University of Medicine and Dentistry of New Jersey–Robert Wood Johnson Medical School, New Brunswick, New Jersey

Received 13 February 2009 ,Revised 11 March 2009 ,Accepted 12 March 2009.

References 

  1. Adamson D, Baker V. Multiple births from assisted reproductive technologies: a challenge that must be met. Fertil Steril. 2004;81:500–504
  2. Chung Y, Bishop CE, Treff NR, Walker SJ, Sandler VM, Becker S, et al. Reprogramming of human somatic cells using human and animal oocytes. Cloning Stem Cells. Published online February 2, 2009 [Epub ahead of print].
  3. Di X, Matsuzaki H, Webster TA, Hubbell E, Liu G, Dong S, et al. Dynamic model based algorithms for screening and genotyping over 100K SNPs on oligonucleotide microarrays. Bioinformatics. 2005;21:1958–1963
  4. Levy B, Dunn TM, Kaffe S, Kardon N, Hirschhorn K. Clinical applications of comparative genomic hybridization. Genet Med. 1998;1:4–12
  5. Kirchhoff M, Gerdes T, Rose H, Maahr J, Ottesen AM, Lundsteen C. Detection of chromosomal gains and losses in comparative genomic hybridization analysis based on standard reference intervals. Cytometry. 1998;31:163–173
  6. Thornhill AR, Grath JA, Eady RA, Braude P, Handyside AH. A comparison of different lysis buffers to assess allele dropout from single cells from preimplantation genetic diagnosis. Prenat Diagn. 2001;21:490–497
  7. Findlay I, Urquhart A, Quirke P, Sullivan KM, Rutherford A, Lilford R. Simultaneous DNA “fingerprinting,” diagnosis of sex and single-gene defect status from a single cell. Mol Hum Reprod. 1995;1:85–93
  8. Katz MG, Trounson AO, Cram DS. DNA fingerprinting of sister blastomeres from human IVF embryos. Hum Reprod. 2002;17:490–497
  9. Jones GM, Cram DS, Song B, Kokkali G, Pantos K, Trounson AO. Novel strategy with potential to identify developmentally competent IVF blastocysts. Hum Reprod. 2008;23:1748–1759
  10. Coskun S, Alsmadi O. Whole genome amplification from a single cell: a new era for preimplantation genetic diagnosis. Prenat Diagn. 2007;27:297–302
  11. Dixon LA, Murray CM, Archer EJ, Dobbins AE, Koumi P, Gill P. Validation of a 21-locus autosomal SNP multiplex for forensic identification purposes. Forensic Sci Int. 2005;154:62–77
  12. Gill P. An assessment of the utility of single nucleotide polymorphisms (SNPs) for forensic purposes. Int J Legal Med. 2001;114:204–210
  13. Findikli N, Kahraman S, Kumtepe Y, Donmez E, Benkhalifa M, Biricik A, et al. Assessment of DNA fragmentation and aneuploidy on poor quality human embryos. Reprod Biomed Online. 2004;8:196–206
  14. Van Langendonckt A, Demylle D, Wyns C, Nisolle M, Donnez J. Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study. Fertil Steril. 2001;76:1023–1031
  15. Kea B, Gebhardt J, Watt J, Westphal LM, Lathi RB, Milki AA, et al. Effect of reduced oxygen concentrations on the outcome of in vitro fertilization. Fertil Steril. 2007;87:213–216
  16. Katz-Jaffe MG, Schoolcraft WB, Gardner DK. Analysis of protein expression (secretome) by human and mouse preimplantation embryos. Fertil Steril. 2006;86:678–685
  17. Brison DR, Houghton FD, Falconer D, Roberts SA, Hawkhead J, Humpherson PG, et al. Identification of viable embryos in IVF by noninvasive measurement of amino acid turnover. Hum Reprod. 2004;19:2319–2324
  18. Scott RT, Seli E, Miller K, Sakkas D, Scott K, Burns DH. Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study. Fertil Steril. 2008;90:77–83

 N.T., K.M., and R.S. are supported by grants from EMD Serono, Schering Plough, Ferring Pharmaceuticals, and Barr Pharmaceuticals. The spouse of J.S. is an employee of GE Healthcare. B.L. is a consultant and advisory board member for Affymetrix. X.T. has nothing to disclose.

PII: S0015-0282(09)00637-2

doi: 10.1016/j.fertnstert.2009.03.067

Fertility and Sterility
Volume 94, Issue 2 , Pages 477-484 , July 2010

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