Fertility and Sterility
Volume 92, Issue 6 , Pages 2109-2112, December 2009

Processing of semen can result in increased sperm DNA fragmentation

  • Estefanía Toro, M.S.

      Affiliations

    • Institut Marquès, Service of Reproduction, Barcelona, Spain
    • ,
  • Silvia Fernández, M.S.

      Affiliations

    • Institut Marquès, Service of Reproduction, Barcelona, Spain
    • Center for Embryo Medicine, Barcelona and Madrid, Spain
    • ,
  • Ana Colomar, M.S.

      Affiliations

    • Institut Marquès, Service of Reproduction, Barcelona, Spain
    • ,
  • Aïda Casanovas, M.S.

      Affiliations

    • Institut Marquès, Service of Reproduction, Barcelona, Spain
    • ,
  • Juan G. Álvarez, M.D., Ph.D.

      Affiliations

    • Institut Marquès, Service of Reproduction, Barcelona, Spain
    • Fundación Leonardo Marquès, Barcelona, Spain
    • ,
  • Marisa López-Teijón, M.D.

      Affiliations

    • Institut Marquès, Service of Reproduction, Barcelona, Spain
    • Fundación Leonardo Marquès, Barcelona, Spain
    • Center for Embryo Medicine, Barcelona and Madrid, Spain
    • ,
  • Esther Velilla, Ph.D.

      Affiliations

    • Institut Marquès, Service of Reproduction, Barcelona, Spain
    • Center for Embryo Medicine, Barcelona and Madrid, Spain
    • Corresponding Author InformationReprint requests: Esther Velilla, Ph.D., Reproduction Unit, Institut Marques, Manuel Girona 33, 08034 Barcelona, Spain (FAX: 34-93-285-82-16).

Received 12 February 2009; received in revised form 22 May 2009; accepted 27 May 2009. published online 15 July 2009.

Processing of semen for assisted reproductive technologies (ART) entails a number of procedures that include semen liquefaction, removal of seminal plasma by centrifugation, incubation, and cryopreservation. The results of this study indicate that incubation of semen at room temperature and semen cryopreservation can result in increased levels of sperm DNA fragmentation.

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 E.T. has nothing to disclose. S.F. has nothing to disclose. A.C. has nothing to disclose. A.C. has nothing to disclose. J.G.A. has nothing to disclose. M.L.-T. has nothing to disclose. E.V. has nothing to disclose.

PII: S0015-0282(09)01206-0

doi:10.1016/j.fertnstert.2009.05.059

Fertility and Sterility
Volume 92, Issue 6 , Pages 2109-2112, December 2009

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