Fertility and Sterility
Volume 93, Issue 1 , Pages 307-310, 1 January 2010

Ovarian tissue thawing: A comparison of two conditions

  • Anne Perdrix, M.D.

      Affiliations

    • Reproductive Biology Laboratory-Centre D;Etude et de Conservation des Deufs et du Sperde, Rouen University Hospital, Institute for Biomedical Research, University of Rouen, Rouen, France
  • ,
  • Bertrand Macé, Ph.D.

      Affiliations

    • Reproductive Biology Laboratory-Centre D;Etude et de Conservation des Deufs et du Sperde, Rouen University Hospital, Institute for Biomedical Research, University of Rouen, Rouen, France
  • ,
  • Jean-Pierre Milazzo, S.C.

      Affiliations

    • Reproductive Biology Laboratory-Centre D;Etude et de Conservation des Deufs et du Sperde, Rouen University Hospital, Institute for Biomedical Research, University of Rouen, Rouen, France
  • ,
  • Agnès Liard-Zmuda, Ph.D.

      Affiliations

    • Paediatric Surgery, Rouen University Hospital, Institute for Biomedical Research, University of Rouen, Rouen, France
  • ,
  • Marc Baron, M.D.

      Affiliations

    • Department of Surgery, Becquerel Cancer Center, University of Rouen, Rouen, France
  • ,
  • Nathalie Rives, Ph.D.

      Affiliations

    • Reproductive Biology Laboratory-Centre D;Etude et de Conservation des Deufs et du Sperde, Rouen University Hospital, Institute for Biomedical Research, University of Rouen, Rouen, France
    • Centre de Recherche Clinique Institut National de la Sante et de la Recherche Medicale (INSERM), Institute for Biomedical Research, University of Rouen, Rouen, France
    • Corresponding Author InformationReprint requests: Professor Nathalie Rives, Reproductive Biology Laboratory-CECOS, Rouen University Hospital, 1 rue de Germont, 76031 Rouen Cedex, France (FAX: 2-35-982007).

Received 22 January 2009; received in revised form 1 July 2009; accepted 6 July 2009. published online 14 August 2009.

Two different protocols to thaw cryopreserved human ovarian cortex have been evaluated using a histological analysis. The slower one, based on a progressive dilution of cryoprotectants, seems to maintain an optimal follicle morphology.

Key Words: Cryopreservation, human follicle, ovarian tissue, thawing conditions

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 A.P. has nothing to disclose. B.M. has nothing to disclose. J.-P.M. has nothing to disclose. A.L.-Z. has nothing to disclose. M.B. has nothing to disclose. N.R. has nothing to disclose.

PII: S0015-0282(09)01436-8

doi:10.1016/j.fertnstert.2009.07.967

Fertility and Sterility
Volume 93, Issue 1 , Pages 307-310, 1 January 2010