Fertility and Sterility
Volume 94, Issue 4 , Pages 1302-1307 , September 2010

Mechanically expanding the zona pellucida of human frozen thawed embryos: a new method of assisted hatching

  • Cong Fang, Ph.D.
    • ,
  • Tao Li, Ph.D.

      Affiliations

    • Corresponding Author InformationReprint requests: Tao Li, Ph.D., Reproductive Medicine Center, First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou, Guangdong, People's Republic of China (FAX: 8620-87755766-8365).
    • ,
  • Ben-Yu Miao, M.D.
    • ,
  • Guang-Lun Zhuang, M.D.
    • ,
  • Canquan Zhou, M.D.

Received 26 June 2009 ,Revised 7 August 2009 ,Accepted 7 August 2009.

References 

  1. Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, et al. Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod. 1990;5:7–13
  2. Manna C, Rienzi L, Greco E, Sbracia M, Rahman A, Poverini R, et al. Zona pellucida solubility and cortical granule complements in human oocytes following assisted reproductive techniques. Zygote. 2001;9:201–210
  3. Gabrielsen A, Agerholm I, Toft B, Hald F, Petersen K, Aagaard J, et al. Assisted hatching improves implantation rates on cryopreserved–thawed embryos. A randomized prospective study. Hum Reprod. 2004;19:2258–2262
  4. Tucker MJ, Cohen J, Massey JB, Mayer MP, Wiker SR, Wright G. Partial zona dissection of zona pellucida of frozen thawed human embryo may enhance blastocyst hatching, implantation and pregnancy rate. Obstet Gynecol. 1991;165:341–345
  5. Cohen J, Alikani M, Trowbridge J, Rosenwaks Z. Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod. 1992;7:685–691
  6. Fong CY, Bongso A, Ng SC, Kumar J, Trounson A, Ratnam S. Blastocyst transfer after enzymatic treatment of the zona pellucida: improving in-vitro fertilization and understanding implantation. Hum Reprod. 1998;13:2926–2932
  7. Antinori S, Panci C, Selman HA, Caffa B, Dani G, Versaci C. Zona thinning with the use of laser: a new approach to assisted hatching in humans. Hum Reprod. 1996;11:590–594
  8. Balaban B, Urman B, Alatas C, Mercan R, Mumcu A, Isiklar A. A comparison of four different techniques of assisted hatching. Hum Reprod. 2002;17:1239–1243
  9. Sifer C, Sellami A, Poncelet C, Kulski P, Martin-Pont B, Bottero J, et al. A prospective randomized study to assess the benefit of partial zona pellucida digestion before frozen-thawed embryo transfers. Hum Reprod. 2006;21:2384–2389
  10. Primi MP, Senn A, Montag M, Van der Ven H, Mandelbaum J, Veiga A, et al. A European multicentre prospective randomized study to assess the use of assisted hatching with a diode laser and the benefit of an immunosuppressive/antibiotic treatment in different patient populations. Hum Reprod. 2004;19:2325–2333
  11. Frydman N, Madoux S, Hesters L, Duvernoy C, Feyereisen E, Le Du A, et al. A randomized double-blind controlled study on the efficacy of laser zona pellucida thinning on live birth rates in cases of advanced female age. Hum Reprod. 2006;21:2131–2135
  12. Nadir Ciray H, Bener F, Karagenç L, Ulug U, Bahçeci M. Impact of assisted hatching on ART outcome in women with endometriosis. Hum Reprod. 2005;20:2546–2549
  13. Lee DR, Lee JE, Yoon HS, Lee HJ, Kim MK, Roh SI. The supplementation of culture medium with protease improves the hatching rate of mouse embryos. Hum Reprod. 1997;12:2493–2498
  14. Balaban B, Urman B, Yakin K, Isiklar A. Laser-assisted hatching increases pregnancy and implantation rates in cryopreserved embryos that were allowed to cleave in vitro after thawing: a prospective randomized study. Hum Reprod. 2006;21:2136–2140
  15. Ng EH, Naveed F, Lau EY, Yeung WS, Chan CC, Tang OS, et al. A randomized double blind controlled study of the efficacy of laser assisted hatching on implantation and pregnancy rates of frozen–thawed embryo transfer at the cleavage stage. Hum Reprod. 2005;20:979–985
  16. Blake DA, Forsberg AS, Johansson BR, Wikland M. Laser zona pellucida thinning—an alternative approach to assisted hatching. Hum Reprod. 2001;16:1959–1964
  17. Gordon JW, Dapunt U. A new mouse model for embryos with a hatching deficiency and its use to elucidate the mechanism of blastocyst hatching. Fertil Steril. 1993;59:1296–1301
  18. Confino E, Rawlins R, Binor Z, Radwanska E. The effect of the oviduct, uterine, and in vitro environments on zona thinning in the mouse embryo. Fertil Steril. 1997;68:164–167

 C.F. has nothing to disclose. T.L. has nothing to disclose. B-Y.M. has nothing to disclose. G-L.Z. has nothing to disclose. C.Z. has nothing to disclose.

 Supported by Guangdong Natural Science Foundation (No. 9151008901000018/No. 06300778); the National Basic Research Program of China (973 Program, No. 2007CB948103).

 Cong Fang and Tao Li contributed equally to this work.

PII: S0015-0282(09)03477-3

doi: 10.1016/j.fertnstert.2009.08.014

Fertility and Sterility
Volume 94, Issue 4 , Pages 1302-1307 , September 2010

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