Fertility and Sterility
Volume 93, Issue 3 , Pages 1006.e7-1006.e10 , February 2010

Live birth after polar body array comparative genomic hybridization prediction of embryo ploidy—the future of IVF?

  • Simon Fishel, Ph.D.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • Corresponding Author InformationReprint requests: Simon Fishel, Ph.D., CARE Fertility, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham NG8 6PZ, United Kingdom (FAX: 44 115 967 0381).
    • ,
  • Anthony Gordon, Ph.D.

      Affiliations

    • BlueGnome Limited, Cambridge, United Kingdom
    • ,
  • Colleen Lynch, M.Sc.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • Ken Dowell, D.M.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • George Ndukwe, M.B.B.S.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • Ehab Kelada, M.B., B.Ch.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • Simon Thornton, M.D.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • Lucy Jenner, Ph.D.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • Ellen Cater, B.Sc.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • Anthony Brown, Ph.D.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom
    • ,
  • Jose Garcia-Bernardo, Ph.D.

      Affiliations

    • CARE Fertility, Nottingham, United Kingdom

Received 10 July 2009 ,Revised 25 September 2009 ,Accepted 27 September 2009.

References 

  1. Hassold T, Hall H, Hunt P. The original of human aneuploidy: where have we been, where are we going. Hum Mol Genet. 2007;16:203–208
  2. Sher G, Keskintepe L, Keskintepe M, Ginsburg M, Maassarani G, Yakut T, et al. Oocyte karyotyping by comparative genomic hybridization provides a highly reliable method for selecting competent embryos, markedly improving in-vitro fertilisation outcome: a multiphase study. Fertil Steril. 2007;87:1033–1040
  3. Mastenbroek S, Twisk M, van Echten-Arends J, Sikkema-Raddatz B, Korevaar JC, Verhoeve HR, et al. In-vitro fertilization with pre implantation genetic screening. N Engl J Med. 2007;357:9–17
  4. Wilton L. Preimplantation genetic diagnosis for aneuploidy screening in early human embryos: a review. Prenat Diagn. 2002;22:512–518
  5. Verlinsky Y, Cohen J, Munne S, Gianaroli L, Simpson JL, Ferraretti AP, et al. Over a decade of experience with preimplantation genetic diagnosis: a multicenter report. Fertil Steril. 2004;82:292–294
  6. Mastenbroek S, Scriven P, Twisk M, Viville S, Van der Veen F, Repping S. What next for preimplantation genetic screening? More randomised control trials needed?. Hum Reprod. 2008;23:2626–2628
  7. Wells D, Levy B. Cytogenetics in reproductive medicine: the contribution of comparative genomic hybridization (CGH). BioEssays. 2003;25:289–300
  8. Wilton L. Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization. Hum Reprod Update. 2005;11:33–41
  9. Le Caignec C, Spits C, Sermon K, De Rycke M, Thienpont B, Debrock S, et al. Single-cell chromosomal imbalances detection by array CGH. Nucleic Acids Res. 2006;34:e68
  10. Fiegler H, Geigl JB, Langer S, Rigler D, Porter K, Unger K, et al. High resolution array-CGH analysis of single cells. Nucleic Acids Res. 2007;35:e15
  11. Macklon NS, Geraedts JP, Fauser BC. Conception to ongoing pregnancy the “black box” of early pregnancy loss. Hum Reprod Update. 2002;8:333–343
  12. Fritz B, Hallermann C, Olert J, Fuchs B, Bruns M, Aslan M, et al. Cytogenetic analysis of culture failures by comparative genomic hybridization (CGH)—re-evaluation of chromosome aberration rates in early spontaneous abortions. Eur J Hum Genet. 2001;9:539–547
  13. Vanneste E, Voet T, Le Caignec C, Ampe M, Konings P, Melotte C, et al. Chromosome instability is common in human cleavage-stage embryos. Nat Med. 2009;15:577–583
  14. Carter NP. Methods and strategies for analysing copy number variation using DNA micro arrays. Nat Genet. 2007;39:s16–s21
  15. Baldwin EL, Lee JY, Blake DN, Bunke BP, Alexander CR, Kogan AL, et al. Enhanced detection of clinically relevant genomic imbalances using a targeted plus whole genome oligonucleotide microarray. Genet Med. 2008;10:415–429
  16. Staessen C, Platteau P, Van Assche E, Michiels A, Tournaye H, Camus M, et al. Comparison of blastocyst transfer with or without pre implantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: prospective randomised control trial. Hum Reprod. 2004;19:2849–2858
  17. Li M, DeUgarte CM, Surrey M, Danzer H, DeCherney A, Hill DL. Fluorescence in situ hybridization reanalysis of day-6 human blastocysts diagnosed with aneuploidy on day 3. Fertil Steril. 2005;84:1395–1400
  18. Johnson DS, Rabinowitz M, Cinnioglu C, Keller J, Banjevic M, Singer J, et al. Novel technology for simultaneous reliable measurement of multiple alleles and copy number across 24 chromosomes in single human blastomeres. Fertil Steril. 2008;89(Suppl 4):S5
  19. Rabinowitz M, Johnson DS, Salzman J, Banjevic M, Cinnioglu C, Behr B. Reliable concurrent calling of multiple genetic alleles and 24-chromosome ploidy without embryo freezing using parental support technology (PS). Fertil Steril. 2008;90(Suppl. 1):S23
  20. Geraedts J. Training in polar body biopsy in advance of the ESHRE trial. Focus Rep. May 25, 2009;

 S.F. has nothing to disclose. A.G. has nothing to disclose. C.L. has nothing to disclose. K.D. has nothing to disclose. G.N. has nothing to disclose. E.K. has nothing to disclose. S.T. has nothing to disclose. L.J. has nothing to disclose. E.C. has nothing to disclose. A.B. has nothing to disclose. J.G.-B. has nothing to disclose.

PII: S0015-0282(09)03805-9

doi: 10.1016/j.fertnstert.2009.09.055

Fertility and Sterility
Volume 93, Issue 3 , Pages 1006.e7-1006.e10 , February 2010

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