At 0, 2, 4, 8, 16, 24, 48, 72, 96, 168, 336, and 504 hours after cecal abrasion, one to three rats were sacrificed (n = 26). Four nonabraded rats served as controls. Peritoneal adhesion status was evaluated and tissue was collected for histologic and immunohistochemical investigation.
Results
Postoperative tissue attachments were identified as early as 2 hours after cecal abrasion. Significant local edema and vessel congestion appeared within 2 hours, and cellular proliferation was observed at 24 hours; angiogenesis and tissue proliferation remained present at 2 weeks. β1 integrin was highly expressed early and was thereafter decreased. Cellular fibronectin was not detectable until 1 week after cecal abrasion.
Conclusions
Postoperative adhesions are initiated as rapidly as 2 hours after surgical intervention in this rodent model.
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan
Reprint requests: Michael P. Diamond, M.D., Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Wayne State University School of Medicine, 3800 Woodward Avenue, Suite 320-D, Detroit, MI 48201 (TEL: 313-993-4523; FAX: 313-993-4534).
F.D.Y. has nothing to disclose. V.I.S. has nothing to disclose. M.P.D. has performed contract research for Neurocrine, Boehringer Ingelheim, Busante, Ethicon, and Wyeth; been a consultant for Genzyme, Omrix, Neomend, ARC, Baxter and Serono; and member of the board of directors for ARC.
ABSTRACT