Fertility and Sterility
Volume 94, Issue 6 , Pages 2409-2412, November 2010

Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione

  • Gil Ah Kim, M.S.

      Affiliations

    • Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea
    • ,
  • Seung Tae Lee, Ph.D.

      Affiliations

    • Institute of Bioengineering, Swiss Federal Institute of Technology at Lausanne, Lausanne, Switzerland
    • ,
  • Ji Yeon Ahn, Ph.D.

      Affiliations

    • Catholic Institute of Cell Therapy Center, Department of Cellular Medicine, The Catholic University of Korea, Seoul, South Korea
    • ,
  • Jong Heum Park, Ph.D.

      Affiliations

    • Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea
    • ,
  • Jeong Mook Lim, D.V.M., Ph.D.

      Affiliations

    • Stem Cell and Bioevalution, Major in Biomodulation, WCU, and Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea
    • Corresponding Author InformationReprint requests: Jeong Mook Lim, D.V.M., Ph.D., Stem Cell and Bioevaluation Laboratory, Seoul National University, Building 200-#4223, Sillim-9 Dong, Seoul 151-921, South Korea (FAX: +822-874-2555).

Received 28 September 2009; received in revised form 11 January 2010; accepted 27 January 2010. published online 16 March 2010.

Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation.

Key Words: Stem cells, cryopreservation, antioxidant, reactive oxygen species

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 G.A.K. has nothing to disclose. S.T.L. has nothing to disclose. J.Y.A. has nothing to disclose. J.H.P. has nothing to disclose. J.M.L. has nothing to disclose.

 Supported by a grant (SC-5160) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science, and Technology, Republic of Korea. This study was also supported by WCU programs (R31-10056) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology.

PII: S0015-0282(10)00148-2

doi:10.1016/j.fertnstert.2010.01.073

Fertility and Sterility
Volume 94, Issue 6 , Pages 2409-2412, November 2010

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