Fertility and Sterility
Volume 93, Issue 8 , Pages 2764-2766, 15 May 2010

Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

  • Irma C. Oskam, Ph.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Oslo University Hospital, Oslo, Norway
    • Corresponding Author InformationReprint requests: Irma C. Oskam, Ph.D., Sognsvannsveien 20, 0027 Oslo, Norway (FAX: 4723072940).
    • ,
  • Babak A. Asadi, M.Sc.

      Affiliations

    • Department of Obstetrics and Gynecology, Oslo University Hospital, Oslo, Norway
    • ,
  • Regiane R. Santos, Ph.D.

      Affiliations

    • Department of Equine Sciences, Veterinary Pharmaceuticals, Pharmacology and Toxicology Division, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands

Received 18 December 2009; received in revised form 25 January 2010; accepted 1 February 2010. published online 16 March 2010.

Ovarian cortical fragments were frozen in presence of 1.5 M PROH, thawed at different temperatures (37°, 30°, or 4°C) and submitted to histologic and ultrastructural analysis. Thawing at 30°C minimized stromal and follicular damage when compared with 4° and 37°C.

Key Words: Female fertility, cryopreservation, stroma, preantral follicles, 1, 2-propanediol

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 I.C.O. has nothing to disclose. B.A.A. has nothing to disclose. R.R.S. has nothing to disclose.

PII: S0015-0282(10)00247-5

doi:10.1016/j.fertnstert.2010.02.003

Fertility and Sterility
Volume 93, Issue 8 , Pages 2764-2766, 15 May 2010

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