Fertility and Sterility
Volume 93, Issue 8 , Pages 2729-2733 , 15 May 2010

Embryo vitrification affects the methylation of the H19/Igf2 differentially methylated domain and the expression of H19 and Igf2

  • Zengyan Wang, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, People's Republic of China
    • Reproductive Medicine Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China
    • ,
  • Ling Xu, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, People's Republic of China
    • ,
  • Fangfang He, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, People's Republic of China
    • Corresponding Author InformationReprint requests: Fangfang He, M.D., Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, 1 Shuaifuyuan Alley, Dongcheng District, Beijing, People's Republic of China (FAX: 86-010-65296223).

Received 27 August 2009 ,Revised 5 March 2010 ,Accepted 8 March 2010.

References 

  1. Holmes R, Soloway PD. Regulation of imprinted DNA methylation. Cytogenet Genome Res. 2006;113:122–129
  2. Lewis A, Reik W. How imprinting centres work. Cytogenet Genome Res. 2006;113:81–89
  3. Reik W, Constancia M, Fowden A, Anderson N, Dean W, Ferguson-Smith A, et al. Regulation of supply and demand for maternal nutrients in mammals by imprinted genes. J Physiol. 2003;547:35–44
  4. Li T, Vu TH, Ulaner GA, Littman E, Ling JQ, Chen HL, et al. IVF results in de novo DNA methylation and histone methylation at an Igf2-H19 imprinting epigenetic switch. Mol Hum Reprod. 2005;11:631–640
  5. Fauque P, Jouannet P, Lesaffre C, Ripoche MA, Dandolo L, Vaiman D, et al. Assisted reproductive technology affects developmental kinetics, H19 imprinting control region methylation and H19 gene expression in individual mouse embryos. BMC Dev Biol. 2007;7:116
  6. Rivera RM, Stein P, Weaver JR, Mager J, Schultz RM, Bartolomei MS. Manipulations of mouse embryos prior to implantation result in aberrant expression of imprinted genes on day 9.5 of development. Hum Mol Genet. 2008;17:1–14
  7. Park SY, Kim EY, Cui XS, Tae JC, Lee WD, Kim NH, et al. Increase in DNA fragmentation and apoptosis-related gene expression in frozen-thawed bovine blastocysts. Zygote. 2006;14:125–131
  8. Mamo S, Bodo S, Kobolak J, Polgar Z, Tolgyesi G, Dinnyes A. Gene expression profiles of vitrified in vivo derived 8-cell stage mouse embryos detected by high density oligonucleotide microarrays. Mol Reprod Dev. 2006;73:1380–1392
  9. Dhali A, Anchamparuthy VM, Butler SP, Pearson RE, Mullarky IK, Gwazdauskas FC. Gene expression and development of mouse zygotes following droplet vitrification. Theriogenology. 2007;68:1292–1298
  10. Wang Z, Sun Z, Chen Y, He F. A modified cryoloop vitrification protocol in the cryopreservation of mature mouse oocytes. Zygote. 2009;17:217–224
  11. Sun ZY, He FF, Yu Q, Deng CY. The application of vitrification in human blastocyst cryopreservation: Analysis of clinical outcome of 117 cycles. J Reprod Med. 2006;43–47
  12. Mann MR, Chung YG, Nolen LD, Verona RI, Latham KE, Bartolomei MS. Disruption of imprinted gene methylation and expression in cloned preimplantation stage mouse embryos. Biol Reprod. 2003;69:902–914
  13. Kaffer CR, Grinberg A, Pfeifer K. Regulatory mechanisms at the mouse Igf2/H19 locus. Mol Cell Biol. 2001;21:8189–8196
  14. Engel N, Raval AK, Thorvaldsen JL, Bartolomei SM. Three-dimensional conformation at the H19/Igf2 locus supports a model of enhancer tracking. Hum Mol Genet. 2008;17:3021–3029
  15. Eggenschwiler J, Ludwig T, Fisher P, Leighton PA, Tilghman SM, Efstratiadis A. Mouse mutant embryos overexpressing IGF-II exhibit phenotypic features of the Beckwith-Wiedemann and Simpson-Golabi-Behmel syndromes. Genes Dev. 1997;11:3128–3142
  16. Gicquel C, Gaston V, Mandelbaum J, Siffroi JP, Flahault A, Le Bouc Y. In vitro fertilization may increase the risk of Beckwith-Wiedemann syndrome related to the abnormal imprinting of the KCN1OT gene. Am J Hum Genet. 2003;72:1338–1341
  17. Khosla S, Dean W, Brown D, Reik W, Feil R. Culture of preimplantation mouse embryos affects fetal development and the expression of imprinted genes. Biol Reprod. 2001;64:918–926
  18. Mann MR, Lee SS, Doherty AS, Verona RI, Nolen LD, Schultz RM, et al. Selective loss of imprinting in the placenta following preimplantation development in culture. Development. 2004;131:3727–3735
  19. Sasaki H, Ishihara K, Kato R. Mechanisms of Igf2/H19 imprinting: DNA methylation, chromatin and long-distance gene regulation. J Biochem. 2000;127:711–715

 Z.W. has nothing to disclose. L.X. has nothing to disclose. F.H. has nothing to disclose.

 Supported by grant no. 2008BAI57B02 from the Chinese Ministry of Science and Technology.

PII: S0015-0282(10)00462-0

doi: 10.1016/j.fertnstert.2010.03.025

Fertility and Sterility
Volume 93, Issue 8 , Pages 2729-2733 , 15 May 2010

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