Fertility and Sterility
Volume 95, Issue 1 , Pages 105-109, January 2011

Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human sperm

  • Agustín García-Peiró, B.S.

      Affiliations

    • Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
    • Càtedra de Recerca Eugin-UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain
    • Corresponding Author InformationReprint requests: Jordi Benet, Ph.D., and Agustín García-Peiró, B.S., Departament de Biologia Cel·lular, Fisiologia i Immunologia, Unitat de Biologia Cel·lular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain (TEL: 34-93-581-1773; FAX: 34-93-581-1025).
    • ,
  • Juan Martínez-Heredia, Ph.D.

      Affiliations

    • Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
    • ,
  • María Oliver-Bonet, Ph.D.

      Affiliations

    • Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
    • ,
  • Carlos Abad, M.D.

      Affiliations

    • Servei d'Urologia, Sabadell, Spain
    • ,
  • María José Amengual, Ph.D.

      Affiliations

    • UDIAT, Consorci Hospitalari Parc Taulí, Sabadell, Spain
    • ,
  • Joaquima Navarro, Ph.D.

      Affiliations

    • Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
    • ,
  • Celine Jones, B.S.

      Affiliations

    • Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    • ,
  • Kevin Coward, Ph.D.

      Affiliations

    • Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
    • ,
  • Jaime Gosálvez, Ph.D.

      Affiliations

    • Departamento de Biología, Unidad de Genética, Universidad Autónoma de Madrid, Madrid, Spain
    • ,
  • Jordi Benet, Ph.D.

      Affiliations

    • Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
    • Corresponding Author InformationReprint requests: Jordi Benet, Ph.D., and Agustín García-Peiró, B.S., Departament de Biologia Cel·lular, Fisiologia i Immunologia, Unitat de Biologia Cel·lular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain (TEL: 34-93-581-1773; FAX: 34-93-581-1025).

Received 22 April 2010; received in revised form 8 June 2010; accepted 16 June 2010. published online 29 July 2010.

Objective

To investigate the relationship between the protamine 1 to protamine 2 (P1/P2) ratio and the rate of sperm DNA fragmentation in sperm samples from human males with proven fertility and three different cohorts of male patients.

Design

P1/P2 ratio was analyzed using acid-urea polyacrylamide acid-urea gels electrophoresis (PAGE). Sperm DNA fragmentation using sperm chromatin dispersion methodology was analyzed after 0, 4, 8, and 24 hours of incubation at 37°C.

Setting

University medical school and hospital.

Patient(s)

A total of 32 human males: six with proven fertility, seven carriers of chromosome reorganizations, nine clinical varicocele patients, and ten subclinical varicocele patients.

Intervention(s)

None.

Main Outcome Measure(s)

P1/P2 ratio, sperm DNA fragmentation (SDF) and the rate of sperm DNA fragmentation (rSDF).

Result(s)

P1/P2 ratio correlated with SDF and rSDF. Statistical differences were detected between fertile controls and patients for the three pathologies studied. rSDF yielded information that differed from baseline SDF. No differences were detected for P1/P2 ratio among patient groups, in reference to the three pathologies studied.

Conclusion(s)

SDF and rSDF correlates with P1/P2 ratio in human sperm, and statistical differences were detected when fertile controls were compared with three different cohorts of patients.

Key Words: Spermatozoa, protamine, P1/P2 ratio, DNA integrity, infertility, DNA damage

 

 A.G-P. has nothing to disclose. J.M-H. has nothing to disclose. M.O-B. has nothing to disclose. C.A. has nothing to disclose. M.J.A. has nothing to disclose. J.N. has nothing to disclose. C.J. has nothing to disclose. K.C. has nothing to disclose. J.G. has nothing to disclose. J.B. has nothing to disclose.

 A.G-P. and J.M-H. contributed equally to this work.

 Supported by the Fondo de Investigación Sanitaria (FIS) (PI051834, PI080623), Generalitat de Catalunya (2009 SGR 1107), Ministerio de Ciencia y Tecnología (MCYT) (BFU 2007-66340/BFI), and Càtedra de Recerca Eugin-UAB.

PII: S0015-0282(10)01006-X

doi:10.1016/j.fertnstert.2010.06.053

Fertility and Sterility
Volume 95, Issue 1 , Pages 105-109, January 2011

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