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Volume 95, Issue 1 , Pages 105-109 , January 2011

Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human sperm

Agustín García-Peiró, B.S.
- Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
- Càtedra de Recerca Eugin-UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain
- Reprint requests: Jordi Benet, Ph.D., and Agustín García-Peiró, B.S., Departament de Biologia Cel·lular, Fisiologia i Immunologia, Unitat de Biologia Cel·lular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain (TEL: 34-93-581-1773; FAX: 34-93-581-1025).
Juan Martínez-Heredia, Ph.D.
- Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
María Oliver-Bonet, Ph.D.
- Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
Carlos Abad, M.D.
- Servei d'Urologia, Sabadell, Spain
María José Amengual, Ph.D.
- UDIAT, Consorci Hospitalari Parc Taulí, Sabadell, Spain
Joaquima Navarro, Ph.D.
- Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
Celine Jones, B.S.
- Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
Kevin Coward, Ph.D.
- Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
Jaime Gosálvez, Ph.D.
- Departamento de Biología, Unidad de Genética, Universidad Autónoma de Madrid, Madrid, Spain
Jordi Benet, Ph.D.
- Departament de Biologia Cel·lular, Fisiologia i Immunologia, Bellaterra, Spain
- Reprint requests: Jordi Benet, Ph.D., and Agustín García-Peiró, B.S., Departament de Biologia Cel·lular, Fisiologia i Immunologia, Unitat de Biologia Cel·lular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain (TEL: 34-93-581-1773; FAX: 34-93-581-1025).

Received 22 April 2010 ,Revised 8 June 2010 ,Accepted 16 June 2010.

Analysis of protamine 1 (P1) and protamine 2 (P2) ratio. (A) Proteins extracted from spermatozoa, separated on a polyacrylamide-acetic-urea gel and stained with Coomassie blue. Lanes 1–5 and 6–10 corr

Analysis of protamine 1 (P1) and protamine 2 (P2) ratio. (A) Proteins extracted from spermatozoa, separated on a polyacrylamide-acetic-urea gel and stained with Coomassie blue. Lanes 1–5 and 6–10 correspond to different sperm samples from clinical and subclinical varicocele patients, respectively. (B) Example from a fertile donor sample with normal P1/P2 ratio (left) and a patient sample with a highly altered P1/P2 ratio (right).
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Box-and-whisker plots showing (A) differences between control and patients groups for P1/P2 ratio, rSDF, and SDF (t0) and (B) differences between control, rearranged genome, and clinical and subclinic

Box-and-whisker plots showing (A) differences between control and patients groups for P1/P2 ratio, rSDF, and SDF (t0) and (B) differences between control, rearranged genome, and clinical and subclinical varicocele groups for P1/P2 ratio, rSDF, and SDF (t0).
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A.G-P. has nothing to disclose. J.M-H. has nothing to disclose. M.O-B. has nothing to disclose. C.A. has nothing to disclose. M.J.A. has nothing to disclose. J.N. has nothing to disclose. C.J. has nothing to disclose. K.C. has nothing to disclose. J.G. has nothing to disclose. J.B. has nothing to disclose.

A.G-P. and J.M-H. contributed equally to this work.

Supported by the Fondo de Investigación Sanitaria (FIS) (PI051834, PI080623), Generalitat de Catalunya (2009 SGR 1107), Ministerio de Ciencia y Tecnología (MCYT) (BFU 2007-66340/BFI), and Càtedra de Recerca Eugin-UAB.

PII: S0015-0282(10)01006-X

doi: 10.1016/j.fertnstert.2010.06.053

« Previous Next » Fertility and Sterility
Volume 95, Issue 1 , Pages 105-109 , January 2011