Fertility and Sterility
Volume 95, Issue 1 , Pages 363-365 , January 2011

SEPT12 deficiency causes sperm nucleus damage and developmental arrest of preimplantation embryos

  • Ying-Hung Lin, Ph.D.

      Affiliations

    • Graduate Institute of Basic Medical Sciences, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • Department of Obstetrics and Gynecology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • ,
  • Chuan-Kai Chou, M.Sc.

      Affiliations

    • National Laboratory Animal Center, Taipei, Taiwan
    • ,
  • Yu-Ching Hung, M.Sc.

      Affiliations

    • National Laboratory Animal Center, Taipei, Taiwan
    • ,
  • I-Shing Yu, Ph.D.

      Affiliations

    • Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
    • ,
  • Hsien-An Pan, M.D.

      Affiliations

    • Department of Obstetrics and Gynecology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • ,
  • Shu-Wha Lin, Ph.D.

      Affiliations

    • Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
    • ,
  • Pao-Lin Kuo, M.D.

      Affiliations

    • Graduate Institute of Basic Medical Sciences, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • Department of Obstetrics and Gynecology, National Cheng Kung University, College of Medicine, Tainan, Taiwan
    • Corresponding Author InformationReprint requests: Pao-Lin Kuo, M.D., Division of Genetics, Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, 138 Sheng-Li Road, Tainan, Taiwan 704 (FAX: 886-6-276-6185).

Received 16 March 2010 ,Revised 2 July 2010 ,Accepted 12 July 2010.

  • Image Result

    Sperm nuclear DNA status of Septin12+/– chimeric mice and SEPT12 expression pattern during human spermiogenesis. (A) Spermatozoa were stained with AO (a), TB (b) and AB (c) dyes. (a) Spermatozoa with

    Sperm nuclear DNA status of Septin12+/– chimeric mice and SEPT12 expression pattern during human spermiogenesis. (A) Spermatozoa were stained with AO (a), TB (b) and AB (c) dyes. (a) Spermatozoa with a normal (green) or abnormal nucleus (yellow). (b, c) Spermatozoa with a normal (light blue) or abnormal (blue) nucleus. (d-f) Spermatozoa isolated from the Septin 12+/-chimeric mice (n = 7) have an increased rate of nuclear DNA damage, compared with wild type (n = 3). ∗P < 0.05; ∗∗∗P < 0.0001. (B) SEPT12 signals during human spermiogenesis. SEPT12 (green), acrosome (red); nucleus (blue). The scale bar represents 5 μm.

 Y-H.L. has nothing to disclose. C-K.C. has nothing to disclose. Y-C.H. has nothing to disclose. I-S.Y. has nothing to disclose. H-A.P. has nothing to disclose. S-W.L. has nothing to disclose. P-L.K. has nothing to disclose.

 Supported by grants from the National Science Council of the Republic of China (NSC96-2314-B-006-003; NSC97-2622-B-006-002-CC1; NSC98-2622-B-006-004- CC1).

PII: S0015-0282(10)02170-9

doi: 10.1016/j.fertnstert.2010.07.1064

Fertility and Sterility
Volume 95, Issue 1 , Pages 363-365 , January 2011

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