Peroxisome proliferator-activated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition

(A) Protein expression of follicular StAR from control, eCG-treated, and eCG+DHEA-treated rats, a representative Western blot, and the graph corresponding to the integrated optical density of the bands. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b, a vs. c, and b vs. c. ∗P < 0.0001 by analysis of variance. (B) MRNA expression of StAR of the same three groups and the graph of integrated optical density bands. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < 0.001 was significantly different from control value by analysis of variance. (C) Protein expression of follicular PPARγ from control, eCG-treated, and eCG+DHEA-treated rats. A representative Western blot and the corresponding graph is shown. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < 0.0001 was significantly different from control value by analysis of variance. (D) The mRNA expression of PPARγ of the three groups and the corresponding graph. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b and a vs. c, P < 0.001; b vs. c, P < 0.05 by analysis of variance. (E) A representative dot plot and the quantitative estimation of viability and apoptosis. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. c, P < 0.05; b vs. d, P < 0.001 by analysis of variance. (F) Agarose gel showing DNA fragmentation and the quantitative estimation of DNA cleavage. Data points represent the mean ± SEM of four independent gel runs: a vs. b, P < 0.01.

(A) Protein expression of follicular StAR from control, eCG-treated, and eCG+DHEA-treated rats, a representative Western blot, and the graph corresponding to the integrated optical density of the bands. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b, a vs. c, and b vs. c. ∗P < 0.0001 by analysis of variance. (B) MRNA expression of StAR of the same three groups and the graph of integrated optical density bands. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < 0.001 was significantly different from control value by analysis of variance. (C) Protein expression of follicular PPARγ from control, eCG-treated, and eCG+DHEA-treated rats. A representative Western blot and the corresponding graph is shown. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < 0.0001 was significantly different from control value by analysis of variance. (D) The mRNA expression of PPARγ of the three groups and the corresponding graph. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b and a vs. c, P < 0.001; b vs. c, P < 0.05 by analysis of variance. (E) A representative dot plot and the quantitative estimation of viability and apoptosis. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. c, P < 0.05; b vs. d, P < 0.001 by analysis of variance. (F) Agarose gel showing DNA fragmentation and the quantitative estimation of DNA cleavage. Data points represent the mean ± SEM of four independent gel runs: a vs. b, P < 0.01.