Intracytoplasmic sperm injection increased fertilization and good-quality embryo formation in patients with non–male factor indications for in vitro fertilization: a prospective randomized study1


      Objective: To compare the fertilization rate and formation of good-quality embryos with conventional IVF and ICSI in patients with non–male factor infertility.
      Design: Prospective controlled study.
      Setting: Infertility clinic.
      Patient(s): Thirty-five patients with non–male factor infertility.
      Intervention(s): Retrieved sibling oocytes were randomly assigned to conventional IVF or ICSI. Of sibling oocytes assigned to ICSI, only metaphase II oocytes were injected with sperm.
      Main Outcome Measure(s): Fertilization rate and formation of good-quality embryos per retrieved oocyte.
      Result(s): Per retrieved oocyte, ICSI resulted in better fertilization rate compared with conventional IVF (71.3% [134 of 188] vs. 57.2% [107 of 187]). Per retrieved oocyte, ICSI also resulted in better formation of good-quality embryos at 48 hours after retrieval compared with conventional IVF (64.4% [121 of 188] vs. 47.1% [88 of 187]).
      Conclusion(s): In IVF patients with non–male factor infertility, subjecting some sibling oocytes to ICSI increased the fertilization rate and formation of good-quality embryos per retrieved oocyte. It also avoided the problem of total fertilization failure in almost all cases.


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