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Ovarian follicular volume and follicular surface area are more accurate indicators of follicular growth and maturation, respectively, than follicular diameter alone
      Serial ultrasonography monitors diameters of individual follicles for growth, whereas serial blood sampling monitors serum estradiol-17β (E2) for overall functional maturation. However, the two-dimensional follicular diameter cannot accurately reflect the three-dimensional growth, and serial measurement of serum concentrations of E2 monitors the overall functional maturation of all, but not of individual, follicles. The objective was to develop accurate and non-invasive methods to monitor the three-dimensional growth and maturation (E2 production) of individual follicles. An accurate method to monitor the three-dimensional follicular growth is to monitor the three-dimensional follicular volume. An accurate, non-invasive method to monitor maturation (E2 production) of an individual follicle is to monitor the follicular surface area where E2-precursor (testosterone)-producing theca interna cells are located.
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