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Characterization of the human microbiome has benefited from the application of powerful
molecular tools utilizing the unique 16S ribosomal subunit’s hypervariable regions
to greatly increase sensitivity. The microbiome of the lower genital tract can prognosticate
obstetrical outcome while the upper reproductive tract remains poorly characterized.
Issues outside of obstetrics are not well studied. Indeed, the microbiome at the time
of embryo transfer has only been evaluated utilizing culture based technology which
is known to capture only a fraction of dominant and major organisms. This study analyzes
the microbiome at the time of single embryo transfer and characterizes the microbiome
by reproductive outcome.
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