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Sperm aneuploidy after testicular cancer treatment: data from a prospective multicenter study performed within the French Centre d’Étude et de Conservation des Oeufs et du Sperme network

  • Nathalie Rives
    Correspondence
    Reprint requests: Nathalie Rives, M.D., Laboratoire de Biologie de la Reproduction–CECOS, EA 4308 “Gamétogenèse et Qualité du Gamète”, Hôpital Charles Nicolle, 1 rue de Germont, Rouen cedex 76031, France.
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Laboratoire de Biologie de la Reproduction–CECOS, CHU–Hôpitaux de Rouen and EA 4308 “Gamétogenèse et Qualité du Gamète,” Université de Rouen, Rouen, France
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  • Marie Walschaerts
    Affiliations
    Université de Toulouse, UPS, Groupe de Recherche en Fertilité Humaine (EA 3694, Human Fertility Research Group), and CECOS, University Hospital, Toulouse, France
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  • Véronique Setif
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Laboratoire de Biologie de la Reproduction–CECOS, CHU–Hôpitaux de Rouen and EA 4308 “Gamétogenèse et Qualité du Gamète,” Université de Rouen, Rouen, France
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  • Sylvianne Hennebicq
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Laboratoire d’Aide à la Procréation–CECOS, Laboratoire AGIM, CNRS-FRE3405, Equipe Génétique-Infertilité-Thérapeutique, Faculté de Médecine de Grenoble, Grenoble, France
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  • Jacqueline Saias
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Laboratoire de Biologie de la Reproduction–CECOS, Hôpital la Conception, Marseille, France
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  • Florence Brugnon
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Assistance Médicale à la Procréation, CECOS, CHU Estaing, and Laboratoire Génétique Reproduction et Développement, GReD, UMR CNRS 6293, INSERM U1103, Faculté de Médecine, Université d’Auvergne, Clermont-Ferrand, France
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  • Jacques Auger
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Département d’Histologie-Embryologie, Biologie de la Reproduction–CECOS, Site Port-Royal, Paris Centre University Hospitals, Paris, France
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  • Isabelle Berthaut
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Service d’Histologie, Biologie de la Reproduction–CECOS, Hôpital Tenon, Paris, France
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  • Ethel Szerman
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Unité de BDR–CECOS, Pole de Biologie, CHU Côte de Nacre, Caen, France
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  • Myriam Daudin
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Université de Toulouse, UPS, Groupe de Recherche en Fertilité Humaine (EA 3694, Human Fertility Research Group), and CECOS, University Hospital, Toulouse, France
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  • Louis Bujan
    Affiliations
    Fédération Française des Centre d’Etude et de Conservation des Œufs et du Sperme Humains (CECOS), Paris, France

    Université de Toulouse, UPS, Groupe de Recherche en Fertilité Humaine (EA 3694, Human Fertility Research Group), and CECOS, University Hospital, Toulouse, France
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      Objective

      To study sperm aneuploidy in a population of testicular cancer (TC) patients treated with the use of either bleomycin-etoposide-cisplatin (BEP) chemotherapy or radiotherapy.

      Design

      Multicenter prospective longitudinal study of TC patients analyzed before treatment and after 3, 6, 12, and 24 months (T3–T24).

      Patient(s)

      Fifty-four TC patients and a control group of 10 fertile sperm donors.

      Setting

      University hospital laboratories.

      Intervention(s)

      Routine semen analyses; sperm aneuploidy and diploidy.

      Main Outcome Measure(s)

      Comparison of sperm characteristics and sperm chromosome abnormalities during TC patient follow-up.

      Result(s)

      Semen characteristics recovered pretreatment values 12 months after radiotherapy and 24 months after more than two BEP cycles. A significant increase in sperm disomy YY and XX was observed in the TC group before treatment compared with the control group. After more than two BEP cycles, the mean sperm aneuploidy rate increased significantly at T12 and reached the pretreatment value at T24. After radiotherapy, the mean sperm aneuploidy returned to the pretreatment value at T12. At T24, nearly 40% of TC patients did not recover their pretreatment sperm aneuploidy rate.

      Conclusion(s)

      Genetic counseling of TC patients should include information on the potential elevated risk of aneuploid conceptus from sperm recovered after treatment and the necessity to postpone conception up to ≥12 months after radiotherapy and ≥24 months after more than two BEP chemotherapy cycles. However, few men receiving one or two BEP cycles and some dropouts are the main limitations of this study.

      Key Words

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